Frontiers in Molecular Neuroscience (Dec 2019)
Transient Receptor Potential Cation Channel Subfamily Vanilloid 4 and 3 in the Inner Ear Protect Hearing in Mice
- Shengnan Wang,
- Shengnan Wang,
- Shengnan Wang,
- Shengnan Wang,
- Qiaowei Geng,
- Qiaowei Geng,
- Qiaowei Geng,
- Qiaowei Geng,
- Lifang Huo,
- Lifang Huo,
- Lifang Huo,
- Yirui Ma,
- Yirui Ma,
- Yirui Ma,
- Yirui Ma,
- Yiting Gao,
- Yiting Gao,
- Yiting Gao,
- Yiting Gao,
- Wei Zhang,
- Wei Zhang,
- Wei Zhang,
- Hailin Zhang,
- Hailin Zhang,
- Hailin Zhang,
- Hailin Zhang,
- Ping Lv,
- Ping Lv,
- Ping Lv,
- Ping Lv,
- Zhanfeng Jia,
- Zhanfeng Jia,
- Zhanfeng Jia,
- Zhanfeng Jia
Affiliations
- Shengnan Wang
- Department of Pharmacology, Hebei Medical University, Shijiazhuang, China
- Shengnan Wang
- Center for Innovative Drug Research and Evaluation, Institute of Medical Science and Health, Hebei Medical University, Shijiazhuang, China
- Shengnan Wang
- The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China
- Shengnan Wang
- The Key Laboratory of New Drug Pharmacology and Toxicology, Shijiazhuang, China
- Qiaowei Geng
- Department of Pharmacology, Hebei Medical University, Shijiazhuang, China
- Qiaowei Geng
- Center for Innovative Drug Research and Evaluation, Institute of Medical Science and Health, Hebei Medical University, Shijiazhuang, China
- Qiaowei Geng
- The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China
- Qiaowei Geng
- The Key Laboratory of New Drug Pharmacology and Toxicology, Shijiazhuang, China
- Lifang Huo
- Department of Pharmacology, Institute of Chinese Integrative Medicine, Hebei Medical University, Shijiazhuang, China
- Lifang Huo
- Center for Innovative Drug Research and Evaluation, Institute of Medical Science and Health, Hebei Medical University, Shijiazhuang, China
- Lifang Huo
- The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China
- Yirui Ma
- Department of Pharmacology, Hebei Medical University, Shijiazhuang, China
- Yirui Ma
- Center for Innovative Drug Research and Evaluation, Institute of Medical Science and Health, Hebei Medical University, Shijiazhuang, China
- Yirui Ma
- The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China
- Yirui Ma
- The Key Laboratory of New Drug Pharmacology and Toxicology, Shijiazhuang, China
- Yiting Gao
- Department of Pharmacology, Hebei Medical University, Shijiazhuang, China
- Yiting Gao
- Center for Innovative Drug Research and Evaluation, Institute of Medical Science and Health, Hebei Medical University, Shijiazhuang, China
- Yiting Gao
- The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China
- Yiting Gao
- The Key Laboratory of New Drug Pharmacology and Toxicology, Shijiazhuang, China
- Wei Zhang
- Department of Pharmacology, Institute of Chinese Integrative Medicine, Hebei Medical University, Shijiazhuang, China
- Wei Zhang
- Center for Innovative Drug Research and Evaluation, Institute of Medical Science and Health, Hebei Medical University, Shijiazhuang, China
- Wei Zhang
- The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China
- Hailin Zhang
- Department of Pharmacology, Hebei Medical University, Shijiazhuang, China
- Hailin Zhang
- Center for Innovative Drug Research and Evaluation, Institute of Medical Science and Health, Hebei Medical University, Shijiazhuang, China
- Hailin Zhang
- The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China
- Hailin Zhang
- The Key Laboratory of New Drug Pharmacology and Toxicology, Shijiazhuang, China
- Ping Lv
- Department of Pharmacology, Hebei Medical University, Shijiazhuang, China
- Ping Lv
- Center for Innovative Drug Research and Evaluation, Institute of Medical Science and Health, Hebei Medical University, Shijiazhuang, China
- Ping Lv
- The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China
- Ping Lv
- The Key Laboratory of New Drug Pharmacology and Toxicology, Shijiazhuang, China
- Zhanfeng Jia
- Department of Pharmacology, Hebei Medical University, Shijiazhuang, China
- Zhanfeng Jia
- Center for Innovative Drug Research and Evaluation, Institute of Medical Science and Health, Hebei Medical University, Shijiazhuang, China
- Zhanfeng Jia
- The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China
- Zhanfeng Jia
- The Key Laboratory of New Drug Pharmacology and Toxicology, Shijiazhuang, China
- DOI
- https://doi.org/10.3389/fnmol.2019.00296
- Journal volume & issue
-
Vol. 12
Abstract
The transient receptor potential cation channel, vanilloid type (TRPV) 3, is a member of the TRPV subfamily that is expressed predominantly in the skin, hair follicles, and gastrointestinal tract. It is also distributed in the organ of Corti of the inner ear and colocalizes with TRPV1 or TRPV4, but its role in auditory function is unknown. In the present study, we demonstrate that TRPV3 is expressed in inner hair cells (HCs) but mainly in cochlear outer HCs in mice, with expression limited to the cytoplasm and not detected in stereocilia. We compared the number of HCs as well as distortion product otoacoustic emissions (DPOAE) and auditory brainstem response (ABR) thresholds between TRPV3 knockout (V3KO) and wild-type (V3WT) mice and found that although most mutants (72.3%) had normal hearing, a significant proportion (27.7%) showed impaired hearing associated with loss of cochlear HCs. Compensatory upregulation of TRPV4 in HCs prevented HC damage and kanamycin-induced hearing loss and preserved normal auditory function in most of these mice. Thus, TRPV4 and TRPV3 in cochlear HCs protect hearing in mice; moreover, the results suggest some functional redundancy in the functions of TRPV family members. Our findings provide novel insight into the molecular basis of auditory function in mammals that can be applied to the development of strategies to mitigate hearing loss.
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