Technological aspects of using bacteriophage Ayka to protect soybeans against bacterial wilt

Abstract

The article presents a study of the use of a protector for rhizobial bacteria as a possible preparative form for the Ayka bacteriophage to effectively combat bacterial wilt of soybean, as well as the possibility of phage transport from the soil to soybean plants through the root system. The study showed that the use of the Premax protector, which is part of the Atuva, WS inoculant, ensures a longer preservation of the bacteriophage titer compared to water during storage of the working solution: the bacteriophage titer in the working solution on the 20th day was 7.2% of the initial titer in the variant with the protector and 0.6% in the variant with an aqueous solution. And also during seed treatment: 24.5% of the initial titer in seed washes in the variant with the protector and 0.6% in the variant with an aqueous solution on the 15th day after the start of the experiment. In addition, the effect of phage translocation into soybean plants from the soil was found in case of root damage, as well as in variant with rapid soil moisture fluctuations.