Comparative effectiveness of the ethanol extract and infusion of green tea leaves (Camellia Sinensis L.) as a diuretic in male swiss mice

Abstract

Green tea (Camellia sinensis L.) contains flavonoid compounds and methylxanthine and has proven efficacy as a diuretic. The dosage form is among the factors determining the biological effects of a drug. This experimental study set out to determine the effectiveness of the ethanol extract and infusion of green tea leaves as a diuretic in male Swiss mice. A total of 20 male Swiss mice were divided into four groups receiving different daily treatments: group I (given 1% Na-CMC), group II (furosemide at a dose of 5.2 mg/kg BW), group III (ethanol extract of green tea leaves at a dose 70 mg/kg BW), and group IV (green tea leaf infusion at a dose of 70mg/kg BW). Before the treatment, all groups were given warm water orally (0.4 ml/20g BW), then the effects of diuretics were measured from urine volume for six hours. The data were analyzed statistically by one-way analysis of variance (ANOVA), followed by LSD comparison tests, with p0.05) from that of the green tea leaf infusion (0.739±0.182). In conclusion, green tea leaves can be used as diuretics in two dosage forms, namely extract and infusion.

Keywords

• diuretics
• ethanol extract
• infusion
• green tea leaves