Zhongguo quanke yixue (Jul 2023)
Expression and Significance of Regulatory B-lymphocytes in Peripheral Blood of Patients with Neuromyelitis Optica Spectrum Disorders
Abstract
Background Regulatory B-lymphocytes (Breg) are a group of cells with negative immunoregulatory effect, which have been detected of abnormal expression in the researches of various autoimmune diseases. Neuromyelitis optica spectrum disorders (NMOSD) belong to the category of autoimmune diseases, there are few studies on the expression and significance of Breg in the peripheral blood of patients with NMOSD. Objective To explore the expression and significance of Breg in peripheral blood of patients with NMOSD. Methods A total of 55 patients with NMOSD admitted to the Department of Neurology of the First Affiliated Hospital of Xinjiang Medical University from June 2019 to December 2021 were selected as the NMOSD group, and 50 healthy volunteers in the same period were selected as the control group. The expression of CD19+CD24hiCD38hi Breg and CD19+CD24hiCD27+ Breg in peripheral blood of patients in the two groups was detected using flow cytometry, the levels of interleukin 10 (IL-10), interleukin 35 (IL-35) and transforming growth factor β1 (TGF-β1) in the serum of patients in the two groups were compared and measured by enzyme-linked immunosorbent assay (ELISA) . Results The proportions of CD19+CD24hiCD38hi Breg and CD19+CD24hiCD27+ Breg cell phenotypes were lower in patients in the NMOSD group than in the control group (t=6.235, 12.131, P<0.05). The levels of IL-10, IL-35 and TGF-β1 were lower in patients in the NMOSD group than in the control group (t=4.249, 7.316, 2.057, P<0.05) . Conclusion The reduced expression of Breg and their cytokines in patients with NMOSD suggests a role for negative immune regulation in the development and progression of NMOSD disease.
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