EXTRACTION AND IDENTIFICATION OF ATROPIN FROM DATURA INNOXIA AND STUDYING EFFECT ON SOME ENZYMES.

Abstract

The work included extraction and identification of atropine.The alkaloid of atropine was extracted from Datura Innoxia using water extraction method.The purification of atropine was accomplished using several chromatographic Thin Layer chromatography (TLC),HPLC method and spectroscopic methods (UV,IR) The results obtained above are compatable with atropine structural formula.The enzymetic activity study of atropine extract have been carried out against acid phosphatase enzyme (ACP), the result obtained medicated that شtropine showed good activition against (ACP) enzyme. The presence of atropine extract showed lowering the concentration of Uric acid , Urea and Sugar in the blood serum. Finlly,the atropine extracted was evaluated for its antibacterial activity against Gram positive (G+) (Staphylococcus aureus,Lactobacillus) and Gram negative (G-) (Escherechia Coli , Proteusral), the result obtained indicated that atropine extract exhibited reasonable antibacterial activity