Production of Autotetraploids in Augmelon (Akebia trifoliata) via Colchicine Induction
Yongle Zhang,
Peng Fu,
Jie Li,
Huai Yang,
Xioxiao Yi,
Zujun Yang,
Chen Chen,
Feiquan Tan,
Jinliang Shen,
Peigao Luo
Affiliations
Yongle Zhang
Provincial Key Laboratory for Plant Genetics and Breeding, College of Agronomy, Sichuan Agricultural University, Chengdu 611134, China
Peng Fu
Provincial Key Laboratory for Plant Genetics and Breeding, College of Agronomy, Sichuan Agricultural University, Chengdu 611134, China
Jie Li
Rice Research Institute, Sichuan Agricultural University, 211, Huimin Road, Wenjiang District, Chengdu 611130, China
Huai Yang
Provincial Key Laboratory for Plant Genetics and Breeding, College of Agronomy, Sichuan Agricultural University, Chengdu 611134, China
Xioxiao Yi
Provincial Key Laboratory for Plant Genetics and Breeding, College of Agronomy, Sichuan Agricultural University, Chengdu 611134, China
Zujun Yang
Center for Information in Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, 2006 Xiyuan Avenue in West Hi-Tech Zone, 611731, Chengdu, Sichuan Province, China
Chen Chen
Provincial Key Laboratory for Plant Genetics and Breeding, College of Agronomy, Sichuan Agricultural University, Chengdu 611134, China
Feiquan Tan
Provincial Key Laboratory for Plant Genetics and Breeding, College of Agronomy, Sichuan Agricultural University, Chengdu 611134, China
Jinliang Shen
College of Forestry, Sichuan Agricultural University, 211, Huimin Road, Wenjiang District, Chengdu 611130, China
Peigao Luo
Provincial Key Laboratory for Plant Genetics and Breeding, College of Agronomy, Sichuan Agricultural University, Chengdu 611134, China
Akebia trifoliata, as a new fruit, is becoming competitive and popular in the markets of eastern Asian countries, especially China, because of its nutritional value and health-promoting functions. To ultimately develop seedless varieties, germinating seeds of the A. trifoliata monoembryonic line ‘710’ with ∼1.5-cm-long roots were treated with five different concentrations of colchicine (0.1%, 0.2%, 0.3%, 0.4%, and 0.5%) for four soaking periods (12, 24, 36, and 48 hours). Ploidy level assessments via both flow cytometry and karyotype analysis revealed some autotetraploids and chimeras in surviving seedlings treated with both 0.4% and 0.5% colchicine for 48 h, but the highest autotetraploid (33.3%) and chimera (19.0%) rates were observed in surviving seedlings treated with 0.3% colchicine for a 12-hour soaking period; no autotetraploids or chimeras were detected in plants treated with only water. In addition, we also found that autotetraploid plants usually presented broader and thicker leaves with larger stomas and epidermic cortical cells. Notably, no autotetraploid of A. trifoliata has been reported previously; therefore, both autotetraploids and chimeras are valuable breeding parents for autotriploid seedless varieties and ideal materials for further theoretical studies.