Sensors (Apr 2023)

Absorption/Attenuation Spectral Description of ESKAPEE Bacteria: Application to Seeder-Free Culture Monitoring, Mammalian T-Cell and Bacteria Mixture Analysis and Contamination Description

  • Bruno Wacogne,
  • Marine Belinger Podevin,
  • Naïs Vaccari,
  • Claudia Koubevi,
  • Céline Codjiová,
  • Emilie Gutierrez,
  • Pauline Bourgeois,
  • Lucie Davoine,
  • Marjorie Robert-Nicoud,
  • Alain Rouleau,
  • Annie Frelet-Barrand

DOI
https://doi.org/10.3390/s23094325
Journal volume & issue
Vol. 23, no. 9
p. 4325

Abstract

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Despite numerous innovations, measuring bacteria concentrations on a routine basis is still time consuming and ensuring accurate measurements requires careful handling. Furthermore, it often requires sampling small volumes of bacteria suspensions which might be poorly representative of the real bacteria concentration. In this paper, we propose a spectroscopy measurement method based on a description of the absorption/attenuation spectra of ESKAPEE bacteria. Concentrations were measured with accuracies less than 2%. In addition, mixing the mathematical description of the absorption/attenuation spectra of mammalian T-cells and bacteria allows for the simultaneous measurements of both species’ concentrations. This method allows real-time, sampling-free and seeder-free measurement and can be easily integrated into a closed-system environment.

Keywords