Phytopathogenic <i>Curtobacterium flaccumfaciens</i> Strains Circulating on Leguminous Plants, Alternative Hosts and Weeds in Russia
Anna D. Tokmakova,
Rashit I. Tarakanov,
Anna A. Lukianova,
Peter V. Evseev,
Lyubov V. Dorofeeva,
Alexander N. Ignatov,
Fevzi S.-U. Dzhalilov,
Sergei A. Subbotin,
Konstantin A. Miroshnikov
Affiliations
Anna D. Tokmakova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow 117997, Russia
Rashit I. Tarakanov
Department of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, Moscow 127434, Russia
Anna A. Lukianova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow 117997, Russia
Peter V. Evseev
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow 117997, Russia
Lyubov V. Dorofeeva
All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Prosp. Nauki 5, Pushchino 142290, Russia
Alexander N. Ignatov
Agrobiotechnology Department, Agrarian and Technological Institute, RUDN University, Miklukho-Maklaya Str., 6, Moscow 117198, Russia
Fevzi S.-U. Dzhalilov
Department of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, Moscow 127434, Russia
Sergei A. Subbotin
Center of Parasitology, Severtsov Institute of Ecology and Evolution, Leninsky Prosp., 33, Moscow 119071, Russia
Konstantin A. Miroshnikov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow 117997, Russia
Many bacterial plant pathogens have a broad host range important for their life cycle. Alternate hosts from plant families other than the main (primary) host support the survival and dissemination of the pathogen population even in absence of main host plants. Metabolic peculiarities of main and alternative host plants can affect genetic diversity within and between the pathogen populations isolated from those plants. Strains of Gram-positive bacterium Curtobacterium flaccumfaciens were identified as being causal agents of bacterial spot and wilt diseases on leguminous plants, and other crop and weed plants, collected in different regions of Russia. Their biochemical properties and susceptibility to copper compounds have been found to be relatively uniform. According to conventional PCR assays, all of the isolates studied were categorised as pathovar Curtobacterim flaccumfaciens pv. flaccumfaciens, a pathogen of legumes. However, the strains demonstrated a substantial diversity in terms of virulence on several tested host plants and different phylogenetic relationships were revealed by BOX-PCR and alanine synthase gene (alaS) sequencing.
