Refining hemodynamic correction in in vivo wide-field fluorescent imaging through linear regression analysis
Jing Li,
Fan Yang,
Kathleen Zhang,
Shiqiang Wu,
James Niemeyer,
Mingrui Zhao,
Peijuan Luo,
Nan Li,
Rongxin Li,
Dan Li,
Weihong Lin,
Jyun-you Liou,
Theodore H. Schwartz,
Hongtao Ma
Affiliations
Jing Li
Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China; Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA
Fan Yang
Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China; Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA
Kathleen Zhang
Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA; Stuyvesant High School, New York, New York 10282, USA
Shiqiang Wu
Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA; Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China
James Niemeyer
Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA
Mingrui Zhao
Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA
Peijuan Luo
Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China; Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA
Nan Li
Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China
Rongxin Li
Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China
Dan Li
Department of Radiology, The First Hospital of Jilin University, Changchun, Jilin 130021, China
Weihong Lin
Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China; Corresponding authors.
Jyun-you Liou
Department of Anesthesiology, Weill Cornell Medicine, New York, New York, NY 10065, USA
Theodore H. Schwartz
Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA; Corresponding authors.
Hongtao Ma
Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA; Corresponding authors.
Accurate interpretation of in vivo wide-field fluorescent imaging (WFFI) data requires precise separation of raw fluorescence signals into neural and hemodynamic components. The classical Beer-Lambert law-based approach, which uses concurrent 530-nm illumination to estimate relative changes in cerebral blood volume (CBV), fails to account for the scattering and reflection of 530-nm photons from non-neuronal components leading to biased estimates of CBV changes and subsequent misrepresentation of neural activity. This study introduces a novel linear regression approach designed to overcome this limitation. This correction provides a more reliable representation of CBV changes and neural activity in fluorescence data. Our method is validated across multiple datasets, demonstrating its superiority over the classical approach.
