Frontiers in Plant Science (Feb 2022)
PpNUDX8, a Peach NUDIX Hydrolase, Plays a Negative Regulator in Response to Drought Stress
- HuaJie He,
- HuaJie He,
- HuaJie He,
- YuZheng Zhang,
- YuZheng Zhang,
- YuZheng Zhang,
- BinBin Wen,
- BinBin Wen,
- BinBin Wen,
- XiangGuang Meng,
- XiangGuang Meng,
- XiangGuang Meng,
- Ning Wang,
- Ning Wang,
- Ning Wang,
- MingYun Sun,
- MingYun Sun,
- MingYun Sun,
- Rui Zhang,
- Rui Zhang,
- Rui Zhang,
- XueHui Zhao,
- XueHui Zhao,
- XueHui Zhao,
- QiuPing Tan,
- QiuPing Tan,
- QiuPing Tan,
- QiuPing Tan,
- Wei Xiao,
- Wei Xiao,
- Wei Xiao,
- DongMei Li,
- DongMei Li,
- DongMei Li,
- XiLing Fu,
- XiLing Fu,
- XiLing Fu,
- XiuDe Chen,
- XiuDe Chen,
- XiuDe Chen,
- Ling Li,
- Ling Li,
- Ling Li
Affiliations
- HuaJie He
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- HuaJie He
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- HuaJie He
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- YuZheng Zhang
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- YuZheng Zhang
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- YuZheng Zhang
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- BinBin Wen
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- BinBin Wen
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- BinBin Wen
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- XiangGuang Meng
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- XiangGuang Meng
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- XiangGuang Meng
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- Ning Wang
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- Ning Wang
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- Ning Wang
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- MingYun Sun
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- MingYun Sun
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- MingYun Sun
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- Rui Zhang
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- Rui Zhang
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- Rui Zhang
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- XueHui Zhao
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- XueHui Zhao
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- XueHui Zhao
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- QiuPing Tan
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- QiuPing Tan
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- QiuPing Tan
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- QiuPing Tan
- College of Life Sciences, Shandong Agricultural University, Taian, China
- Wei Xiao
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- Wei Xiao
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- Wei Xiao
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- DongMei Li
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- DongMei Li
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- DongMei Li
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- XiLing Fu
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- XiLing Fu
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- XiLing Fu
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- XiuDe Chen
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- XiuDe Chen
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- XiuDe Chen
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- Ling Li
- College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China
- Ling Li
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
- Ling Li
- Shandong Province Collaborative Innovation Center for High-Quality and High-Efficiency Vegetable Production, Taian, China
- DOI
- https://doi.org/10.3389/fpls.2021.831883
- Journal volume & issue
-
Vol. 12
Abstract
Drought stress is a serious abiotic stress source that affects the growth and fruit quality of peach trees. However, the molecular mechanism of the NUDIX hydrolase family in peaches in response to drought stress is still unclear. Here, we isolated and identified the PpNUDX8 (Prupe.5G062300.1) gene from the peach NUDIX hydrolase family, and found that PpNUDX8 has a typical NUDIX hydrolase domain. In this study, we performed 15% PEG6000 drought treatment on peach seedlings, and qRT–PCR analysis showed that 15% PEG6000 induced the transcription level of PpNUDX8. Overexpression of PpNUDX8 reduced the tolerance of calli to 4% PEG6000 treatment. Compared with wild-type apple calli, PpNUDX8 transgenic apple calli had a lower fresh weight and higher MDA content. After 15% PEG6000 drought treatment, PpNUDX8 transgenic tobacco had a greater degree of wilting and shorter primary roots than Under control conditions. The chlorophyll, soluble protein, and proline contents in the transgenic tobacco decreased, and the MDA content and relative conductivity increased. At the same time, PpNUDX8 negatively regulated ABA signal transduction and reduced the transcriptional expression of stress response genes. In addition, PpNUDX8 was not sensitive to ABA, overexpression of PpNUDX8 reduced the expression of the ABA synthesis-related gene NCED6 and increases the expression of the ABA decomposition-related gene CYP1 in tobacco, which in turn leads to a decrease in the ABA content in tobacco. In addition, Under control conditions, overexpression of PpNUDX8 destroyed the homeostasis of NAD and reduced nicotinamide adenine dinucleotide (NADH) in tobacco. After 15% PEG6000 drought treatment, the changes in NAD and NADH in PpNUDX8 transgenic tobacco were more severe than those in WT tobacco. In addition, PpNUDX8 also interacted with PpSnRk1γ (Prupe.6G323700.1).
Keywords
