Effect of iron dusts on physiological responses of gram seedlings (Cicer arietinum L.) under laboratory conditions

Abstract

A laboratory experiments was conducted for the assessment of physiological and biochemical responses of iron dust under the influence of different pH levels (6.5, 5.0, 3.0) and two concentration of iron dust (0.1 mg and 0.6 mg) with two particle size (100 μm and 300 μm) sprayed on the Cicer arietinum L. seed surface for fifteen day exposure. Observation was made on germination percentage and germination rate, vigour index, % phytotoxicity of root and shoot, chlorophyll, sugar, protein and proline content in both treated and control plant. The present results revealed that the seed color changes to brown under iron stress. The lower germination percentage and germination rate gradually decrease with pH of the medium but both the parameters were not significantly affected by the iron dust. Moreover higher % phytotoxicity was observed under all treatments compared to control and also lower values of this parameter were recorded in shoot than root. The reduction trend in chlorophyll and protein content was recorded at low pH but reverse result was recorded for sugar. Moreover highest proline was recorded under highly acidic condition.

Keywords

• Gram seeds (Cicer arietinum L.)
• pH level
• Iron dust
• Phytotoxicity
• Biochemical constituents