Nature Communications (Oct 2018)
An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
- Huan-Huan Qiao,
- Fang Wang,
- Rong-Gang Xu,
- Jin Sun,
- Ruibao Zhu,
- Decai Mao,
- Xingjie Ren,
- Xia Wang,
- Yu Jia,
- Ping Peng,
- Da Shen,
- Lu-Ping Liu,
- Zhijie Chang,
- Guirong Wang,
- Shao Li,
- Jun-Yuan Ji,
- Qingfei Liu,
- Jian-Quan Ni
Affiliations
- Huan-Huan Qiao
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Fang Wang
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Rong-Gang Xu
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Jin Sun
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Ruibao Zhu
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Decai Mao
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Xingjie Ren
- Department of Neurology, Institute for Human Genetics, University of California San Francisco
- Xia Wang
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Yu Jia
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Ping Peng
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Da Shen
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Lu-Ping Liu
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- Zhijie Chang
- State Key Laboratory of Membrane Biology, School of Medicine and the School of Life Sciences, Tsinghua University
- Guirong Wang
- State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection
- Shao Li
- MTCM-X Center/Bioinformatics Division, Department of Automation, MOE Key Laboratory of Bioinformatics, BNRIST, Tsinghua University
- Jun-Yuan Ji
- Department of Molecular and Cellular Medicine, College of Medicine, Texas A&M Health Science Center
- Qingfei Liu
- School of Pharmaceutical Sciences, Tsinghua University
- Jian-Quan Ni
- Gene Regulatory Lab, School of Medicine, Tsinghua University
- DOI
- https://doi.org/10.1038/s41467-018-06537-y
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 13
Abstract
Drosophila transgenic RNAi can have drawbacks such as false positives and negative results. Here the authors develop a next generation RNAi system with reduced leakiness of expression and simultaneous knockdown.
