Mycobiota of asparagus vigna seeds (Vigna unguiculata (L.) Walp.) on the south of Western Siberia

Abstract

Composition of seed mycobiota of 87 accessions of asparagus vigna (Vigna unguiculata (L.) Walp.) from the N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) and the Bioresource Scientific Collection of the Central Siberian Botanical Garden (CSBG SB RAS) UNU No.USU 440534 have been investigated on 2018-2019. Seeds of accessions were grown on the south of Western Siberia (54 ° N lat. 83 ° E) in the unheated plastic film greenhouse and examined for fungal microbiota in the Central Siberian Botanical Garden and Novosibirsk State Agrarian University (Novosibirsk). The seed infestation of micromycetes was determined according to GOST 12044-93 (similar to common beans) using the methods of mycological analysis and the Petri dish moist chambers, also on Chapek and KDA media. The following pathogens were assigned to the permanent inhabitants of mycocenosis of asparagus vigna seeds: Fusarium spp., Mucor mucedo, Penicillium spp., Rhizopus spp., Aspergillus niger, to the occasional – Alternaria spp. Botritis sinerea and Sclerotinia sclerotiorum occur moderately and intermittently in the samples of vigna seeds.The highest frequency of occurrence and infestation of asparagus vigna seeds were found for Aspergillus niger (min-max: 30-100% and 0-100%, respectively), the minimum for Alternaria spp. (0-10%).