Nature Communications (Dec 2021)
Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography
- Obinna C. Ubah,
- Eric W. Lake,
- Gihan S. Gunaratne,
- Joseph P. Gallant,
- Marie Fernie,
- Austin J. Robertson,
- Jonathan S. Marchant,
- Tyler D. Bold,
- Ryan A. Langlois,
- William E. Matchett,
- Joshua M. Thiede,
- Ke Shi,
- Lulu Yin,
- Nicholas H. Moeller,
- Surajit Banerjee,
- Laura Ferguson,
- Marina Kovaleva,
- Andrew J. Porter,
- Hideki Aihara,
- Aaron M. LeBeau,
- Caroline J. Barelle
Affiliations
- Obinna C. Ubah
- Elasmogen Ltd
- Eric W. Lake
- Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health
- Gihan S. Gunaratne
- Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health
- Joseph P. Gallant
- Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health
- Marie Fernie
- Elasmogen Ltd
- Austin J. Robertson
- Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health
- Jonathan S. Marchant
- Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin
- Tyler D. Bold
- Division of Infectious Diseases and International Medicine, University of Minnesota Medical School
- Ryan A. Langlois
- Center for Immunology, University of Minnesota
- William E. Matchett
- Center for Immunology, University of Minnesota
- Joshua M. Thiede
- Division of Infectious Diseases and International Medicine, University of Minnesota Medical School
- Ke Shi
- Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
- Lulu Yin
- Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
- Nicholas H. Moeller
- Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
- Surajit Banerjee
- Northeastern Collaborative Access Team, Cornell University, Advanced Photon Source
- Laura Ferguson
- Elasmogen Ltd
- Marina Kovaleva
- Elasmogen Ltd
- Andrew J. Porter
- Elasmogen Ltd
- Hideki Aihara
- Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
- Aaron M. LeBeau
- Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health
- Caroline J. Barelle
- Elasmogen Ltd
- DOI
- https://doi.org/10.1038/s41467-021-27611-y
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 12
Abstract
Shark antibodies (Variable New Antigen Receptors, VNARs) are the smallest naturally occurring antibody fragments. Here, the authors screen a VNAR phage display library against the SARS-CoV2 receptor binding domain (RBD) and identify VNARs that neutralize the SARSCoV-2 virus and discuss their mechanisms of viral neutralization.
