Armaghane Danesh Bimonthly Journal (Apr 2014)
Effects of Methanol Extracts of Different Parts of Three Thymus Species on Proliferation of Human Peripheral Blood Mononuclear Cells and HIV-1 Replication
Abstract
Background & aim: A different characteristic of thymus daenensis such as antimicrobial, antioxidant, anti-fungal and anti-viral makes it a suitable medicinal plant. The aim of this study was to evaluate the effects of methanol extracts of roots, stems, leaves and seeds of three Thymus species collected from Goldasht region on human peripheral blood mononuclear cells and human immunodeficiency virus replication. Methods: The present experimental study was conducted under laboratory conditions. Different parts of three Thymus subspecies including, Lance Flvious, Daynnsys thymus, Thymus vulgaris Karmanykvsv were collected and subsequently extracted. Subsequent to sampling from three healthy donors’ mononuclear cells, they were isolated using ficoll. The effects of different concentrations of extract (10, 100, 200, 800 and 1600 g/ml) on human immunodeficiency virus replication and amplification of human mononuclear cells was evaluated by P24 ELISA and MTT assay respectively. The gathered data were analyzed by one-way ANOVA. Results: All the extracts were able to increase peripheral blood mononuclear cells, so that the maximum and maximum effect was related to root and leaves extract respectively. Effective concentration for 50% inhibition of viral replication was obtained as 500 mg/ml for root extracts. Conclusions: Compared to other parts of the thymus daenensis, a root extract increased human peripheral blood mononuclear cell and able to inhibit the replication of human immunodeficiency virus.
