Bio-Protocol (May 2018)

High Dimensional Functionomic Analysis of Human Hematopoietic Stem and Progenitor Cells at a Single Cell Level

  • Thomas Luh,
  • Kimberly Lucero,
  • Wenji Ma,
  • Jaeyop Lee,
  • Yu Zhou,
  • Yufeng Shen,
  • Kang Liu

DOI
https://doi.org/10.21769/BioProtoc.2851
Journal volume & issue
Vol. 8, no. 10

Abstract

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The ability to conduct investigation of cellular transcription, signaling, and function at the single-cell level has opened opportunities to examine heterogeneous populations at unprecedented resolutions. Although methods have been developed to evaluate high-dimensional transcriptomic and proteomic data (relating to cellular mRNA and protein), there has not been a method to evaluate corresponding high-dimensional functionomic data (relating to cellular functions) from single cells. Here, we present a protocol to quantitatively measure the differentiation potentials of single human hematopoietic stem and progenitor cells, and then cluster the cells according to these measurements. High dimensional functionomic analysis of cell potential allows cell function to be linked to molecular mechanisms within the same progenitor population.