Detection of betalactamase production among Staphylococcus aureus isolated from human dental plaques using iodometric and molecular methods

Abstract

Introduction: Resistance to penicillin among Staphylococcus aureus species is very widespread. One of the most important resistance mechanisms toward penicillin in S. aureus is blaZ production. Detection of beta lactamase production is possible using different methods like disk diffusion, iodometric, acidometric and molecular (PCR) methods. The aim of this study was to detection of beta lactamase production among S. aureus isolated from human dental plaque using iodometric and molecular methods. Descriptive statistics were done using ANOVA test and the statistical package, SPSS, Version 15.0. Methods: In this cross sectional study, 40 samples of human dental plaques was collected through forceps. Identification and isolation of S. aureus from samples was carried out using cultural methods, biochemical and molecular tests. Then, antibiotic resistance patterns of studied Staphylococci were determined using Kirby-Bauer method. Also, betalactamase production among the bacteria was evaluated using different iodometric and molecular methods. Results: 30% (12) of studied samples contained Staphylococcus aureus. All Staphylococcus aureus strains had beta lactamase production ability using filter paper iodometric method. Also, all spesies had blaZ gene, which detected by polymerase chain reaction test. The results of Conclusions: The result of this study showed that molecular (PCR) and iodometric (filter paper) methods were more accurate in detection of penicillin resistant S. aureus.  

Keywords

• Staphylococcus aureus
• Antibiotic resistance
• Beta lactamase
• Iodometric
• PCR