Açaí (<i>Euterpe oleracea</i> Mart.) Attenuates Oxidative Stress and Alveolar Bone Damage in Experimental Periodontitis in Rats
Vinicius Ruan Neves dos Santos,
Deborah Ribeiro Frazão,
Railson de Oliveira Ferreira,
Paulo Fernando Santos Mendes,
Daiane Claydes Baia-da-Silva,
Deiweson Souza-Monteiro,
Leonardo Oliveira Bittencourt,
João Daniel Mendonça de Moura,
José Messias Perdigão,
Bruno José Brito Teixeira,
Everton Luiz Pompeu Varela,
Gabriela de Souza Balbinot,
Sandro Percário,
Herve Rogez,
Cassiano Kuchenbecker Rösing,
Fabrício Mezzomo Collares,
Rafael Rodrigues Lima
Affiliations
Vinicius Ruan Neves dos Santos
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
Deborah Ribeiro Frazão
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
Railson de Oliveira Ferreira
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
Paulo Fernando Santos Mendes
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
Daiane Claydes Baia-da-Silva
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
Deiweson Souza-Monteiro
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
Leonardo Oliveira Bittencourt
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
João Daniel Mendonça de Moura
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
José Messias Perdigão
Center for Valorization of Amazonian Bioactive Compounds, College of Biotechnology, Federal University of Pará, Belém 66075-110, PA, Brazil
Bruno José Brito Teixeira
Center for Valorization of Amazonian Bioactive Compounds, College of Biotechnology, Federal University of Pará, Belém 66075-110, PA, Brazil
Everton Luiz Pompeu Varela
Oxidative Stress Research Laboratory and Post-Graduate Program in Biodiversity and Biotecnology of the BIONORTE Network, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
Gabriela de Souza Balbinot
Dental Materials Laboratory, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil
Sandro Percário
Oxidative Stress Research Laboratory and Post-Graduate Program in Biodiversity and Biotecnology of the BIONORTE Network, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
Herve Rogez
Center for Valorization of Amazonian Bioactive Compounds, College of Biotechnology, Federal University of Pará, Belém 66075-110, PA, Brazil
Cassiano Kuchenbecker Rösing
Department of Periodontology, Faculty of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil
Fabrício Mezzomo Collares
Dental Materials Laboratory, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil
Rafael Rodrigues Lima
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil
Açaí (Euterpe oleracea Mart.) juice is rich in phenolic compounds with high antioxidant capacity. It has been observed that the use of antioxidants may be an additional strategy to nonsurgical periodontal therapy as well as to prevent alveolar bone loss. Thus, the objective of this study was to investigate the effects of açaí supplementation on experimental periodontitis in rats. Twenty male Rattus norvegicus (Wistar) rats were assigned into control, açaí, experimental periodontitis, and experimental periodontitis with açaí supplementation groups. Periodontitis was induced by placing ligatures around the lower first molars. Animals in the açaí groups received 0.01 mL/g of clarified açaí juice for 14 days by intragastric gavage. At the end of the experimental period, blood was collected to assess the reduced glutathione (GSH), Trolox equivalent antioxidant capacity (TEAC), and lipid peroxidation (TBARS) levels. Moreover, hemimandibles were analyzed by micro-computed tomography (micro-CT) for alveolar bone loss and bone quality. Açaí supplementation increased blood total antioxidant capacity and decreased lipid peroxidation. It also reduced alveolar bone loss when compared to the experimental periodontitis group. Moreover, clarified açaí per se modulated the oxidative biochemistry and bone microstructure. Thus, açaí may be considered a viable alternative for managing periodontal oxidative stress and preventing alveolar bone loss.
