LncRNA PVT1 alleviates inflammation of airway via regulating miR-214/STAT6 axis in mouse models with asthma

Abstract

Objective To explore the effect of inhibiting the expression of lncRNA PVT1 on airway inflammation response and the effect on the miR-214/STAT6 axis in mice with bronchial asthma. Methods Mice were randomly divided into control group, model group [asthma induced by ovalbumin(OVA)], lncRNA PVT1 inhibitor group and lncRNA PVT1 NC group with 12 mice in each.Hemocytometer was used to count the total number of inflammatory cells, numbers of macrophages, neutrophils and lymphocytes in broncho-alveolar lavage fluid (BALF). ELISA kit was used to detect the concentration of interleukin-4(IL-4), interleukin-5 (IL-5) and interleukin-13 (IL-13) in the BALF supernatant. The kit was used to detect the level of IgE in serum. HE staining was used to observe and score the infiltration of inflammatory cells in lung tissue. RT-qPCR was used to detect the levels of miR-214 and STAT6 mRNA in lung tissue. Western blot was used to detect the expression of STAT6 and p-STAT6 proteins in lung tissue. The dual luciferase reporter gene detection was used to analyze the targeting relationship between lncRNA PVT1 and miR-214. Results Compared with the control group, the cell counting of inflammatory cells, macrophages, neutrophils and lymphocytes, level of IL-4, IL-5, IL-13 in BALF, serum IgE,inflammatory cell infiltration, STAT6 mRNA and protein phosphorylation in lung tissues of mice in the model group were all significantly increased (P＜0.05).While the miR-214 level in lung tissue was significantly reduced (P＜0.05). Compared with the model group and the lncRNA PVT1 NC group, the total number of inflammatory cells, macrophages, neutrophils and lymphocytes, IL-4, IL-5, IL-13 levels in BALF, and serum IgE ,inflammatory cell infiltration, STAT6 mRNA and protein phosphorylation in lung tissues in the lncRNA PVT1 inhibition group were all significantly reduced (P＜0.05). But the miR-214 exprssion in lung tissue was significantly increased(P＜0.05). miR-214 showed a significant targeting relationship with lncRNA PVT1 (P＜0.05). Conclusions Inhibiting lncRNA PVT1 may increase expression of miR-214 ,inhibit the STAT6 phosphorylation pathway and reduce airway inflammation response of mouse with asthma.

Keywords

• lncrna pvt1| asthma| airway inflammation response| mir-214/stat6 axis