Frontiers in Veterinary Science (Jul 2022)
Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China
- Zhiqiang Wu,
- Huanxin Fang,
- Zhouyi Xu,
- Jiamin Lian,
- Jiamin Lian,
- Zi Xie,
- Zi Xie,
- Zhanxin Wang,
- Jianpin Qin,
- Benli Huang,
- Benli Huang,
- Keyu Feng,
- Keyu Feng,
- Xinheng Zhang,
- Xinheng Zhang,
- Xinheng Zhang,
- Xinheng Zhang,
- Wencheng Lin,
- Wencheng Lin,
- Wencheng Lin,
- Wencheng Lin,
- Hongxin Li,
- Hongxin Li,
- Hongxin Li,
- Hongxin Li,
- Weiguo Chen,
- Weiguo Chen,
- Weiguo Chen,
- Weiguo Chen,
- Qingmei Xie,
- Qingmei Xie,
- Qingmei Xie,
- Qingmei Xie
Affiliations
- Zhiqiang Wu
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Huanxin Fang
- Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, China
- Zhouyi Xu
- Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, China
- Jiamin Lian
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Jiamin Lian
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Zi Xie
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zi Xie
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Zhanxin Wang
- Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, China
- Jianpin Qin
- Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, China
- Benli Huang
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Benli Huang
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Keyu Feng
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Keyu Feng
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Xinheng Zhang
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Xinheng Zhang
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Xinheng Zhang
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Xinheng Zhang
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- Wencheng Lin
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Wencheng Lin
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Wencheng Lin
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Wencheng Lin
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- Hongxin Li
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Hongxin Li
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Hongxin Li
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Hongxin Li
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- Weiguo Chen
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Weiguo Chen
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Weiguo Chen
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Weiguo Chen
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qingmei Xie
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qingmei Xie
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Qingmei Xie
- South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, China
- Qingmei Xie
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- DOI
- https://doi.org/10.3389/fvets.2022.842179
- Journal volume & issue
-
Vol. 9
Abstract
Avian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens, resulting in severe economic losses in the poultry industry. This study aimed to monitor and isolate the molecular identity of IBV in broiler flocks with respiratory symptoms in eight provinces of China. In total, 910 samples (oropharyngeal and cloacal mixed swabs) from broiler flocks showed IBV positive rates of 17.6% (160/910) using PCR assay. Phylogenetic analysis of the complete S1 genes of 160 IBV isolates was performed and revealed that QX-type (GI-19), TW-type (GI-7), 4/91-type (GI-13), HN08-type (GI-22),TC07-2-type (GVI-1), and LDT3-type (GI-28) exhibited IBV positive rates of 58.15, 25, 8.12, 1.86, 5.62, and 1.25%. In addition, recombination analyses revealed that the four newly IBV isolates presented different recombination patterns. The CK/CH/JS/YC10-3 isolate likely originated from recombination events between strain YX10 (QX-type) and strain TW2575-98 (TW-type), the pathogenicity of which was assessed, comparing it with strain GZ14 (TW-type) and strain CK/CH/GD/JR07-7 (QX-type). The complete S1 gene data from these isolates indicate that IBV has consistently evolved through genetic recombination or mutation, more likely changing the viral pathogenicity and leading to larger outbreaks in chick populations, in China.
Keywords
