Boron Nitride Doped Polyhydroxyalkanoate/Chitosan Nanocomposite for Antibacterial and Biological Applications
Abdul Mukheem,
Syed Shahabuddin,
Noor Akbar,
Azizi Miskon,
Norazilawati Muhamad Sarih,
Kumar Sudesh,
Naveed Ahmed Khan,
Rahman Saidur,
Nanthini Sridewi
Affiliations
Abdul Mukheem
Department of Maritime Science and Technology, Faculty of Defence Science and Technology, National Defence University of Malaysia, Kuala Lumpur 57000, Malaysia
Syed Shahabuddin
Research Centre for Nano-Materials and Energy Technology (RCNMET), School of Science and Technology, Sunway University, Subang Jaya 47500, Malaysia
Noor Akbar
Department of Biological Sciences, School of Science and Technology, Sunway University, Subang Jaya 47500, Malaysia
Azizi Miskon
Department of Maritime Science and Technology, Faculty of Defence Science and Technology, National Defence University of Malaysia, Kuala Lumpur 57000, Malaysia
Norazilawati Muhamad Sarih
Polymer Research Laboratory, Department of Chemistry, University of Malaya, Faculty of Science, Kuala Lumpur 50603, Malaysia
Kumar Sudesh
Applied Microbiology and Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia
Naveed Ahmed Khan
Department of Biological Sciences, School of Science and Technology, Sunway University, Subang Jaya 47500, Malaysia
Rahman Saidur
Research Centre for Nano-Materials and Energy Technology (RCNMET), School of Science and Technology, Sunway University, Subang Jaya 47500, Malaysia
Nanthini Sridewi
Department of Maritime Science and Technology, Faculty of Defence Science and Technology, National Defence University of Malaysia, Kuala Lumpur 57000, Malaysia
The present research focused on the fabrication of biocompatible polyhydroxyalkanoate, chitosan, and hexagonal boron nitride incorporated (PHA/Ch-hBN) nanocomposites through a simple solvent casting technique. The fabricated nanocomposites were comprehensively characterized by Fourier transform infrared spectroscope (FT-IR), field emission scanning electroscope (FESEM), and elemental mapping and thermogravimetric analysis (TGA). The antibacterial activity of nanocomposites were investigated through time-kill method against multi drug resistant (MDR) microbes such as methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli) K1 strains. In addition, nanocomposites have examined for their host cytotoxicity abilities using a Lactate dehydrogenase (LDH) assay against spontaneously immortalized human keratinocytes (HaCaT) cell lines. The results demonstrated highly significant antibacterial activity against MDR organisms and also significant cell viability as compared to the positive control. The fabricated PHA/Ch-hBN nanocomposite demonstrated effective antimicrobial and biocompatibility properties that would feasibly suit antibacterial and biomedical applications.
