A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines

Abstract

Song et al. describe an immunoassay method, which is a multiplex, fluorescence-barcoded cell lines system that enables high-throughput screening of antibodies binding to large number of protein variants in a single-tube manner. They demonstrate the utility of this tool by assessing binding of different antibodies to a panel of influenza hemagglutinin antigens and to epitope/domain map CCR2 and CCR5 antibodies.