Establishment of IFA technique for detecting ANCA

Abstract

ANCA are classes of antibodies or in reality autoantibodies usually produce in conditions that degradation of neutrophiles occurs. This is led to vascular damage. They are more common in rheumatic diseases such as Rheumatoid Arthritis (RA), systemic Lupus Erythematus (SLE) and vasculitic diseases especially Wegners’ granulomatosis (With specificity and sensitivity as high as 95%). We decided to design IFA-ANCA because this assay has high sensitivity and in with comparison other techniques such as ELISA & RIA is more economic and simple. On the other hand, screening test for determining ANCA is immunofluorescent assay. ANCA test establishment contains: Isolation of neutrophiles, fixation with Ethanol and Formalin, improvement of method and quality control by using of indirect immunofluorescence assay (IFA), 2 staining pattern are observed. Cytoplasmic (C-ANCA) or classic and prinuclear (P-ANCA) pattern. Established ANCA test by inter assay method had 95% reproducibility and 91% precision.

Keywords

• ANCA
• IFA