Nature Communications (Sep 2020)

Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision

  • Rohit Agarwal,
  • Karl E. Duderstadt

DOI
https://doi.org/10.1038/s41467-020-18456-y
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 10

Abstract

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Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100-fold, and detect rare DNA breaks induced by gyrase.