Eco-Friendly CuO/Fe3O4 Nanocomposite synthesis, characterization, and cytotoxicity study
Poonam Dwivedi,
Abdul Malik,
Hafiza Zumra Fatima Hussain,
Indu Jatrana,
Khalid Imtiyaz,
M.M. Alam Rizvi,
Md Mushtaque,
Azhar U. Khan,
Mahboob Alam,
Mohd Rafatullah
Affiliations
Poonam Dwivedi
Department of Chemistry, School of Basic Sciences, Jaipur National University, Jaipur, 302017 (Rajasthan) India
Abdul Malik
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Hafiza Zumra Fatima Hussain
Department of Environmental, Biological and Pharmaceutical Science and Technology (DISTABiF), University of Campania ‘Luigi Vanvitelli’ Via Vivaldi 43, 81100 Caserta, Italy
Indu Jatrana
Department of Chemistry, School of Basic Sciences, Jaipur National University, Jaipur, 302017 (Rajasthan) India
Khalid Imtiyaz
Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India
M.M. Alam Rizvi
Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India
Md Mushtaque
Department of Chemistry, Millat College (A constituent colle ge of Lalit Narayan Mithila University), Darbhanga, Bihar, India
Azhar U. Khan
Department of Chemistry, School of Basic Sciences, Jaipur National University, Jaipur, 302017 (Rajasthan) India
Mahboob Alam
Division of Chemistry and Biotechnology, Dongguk University, 123, Dongdaero, Gyeongju-si 780714, Republic of Korea
Mohd Rafatullah
Environmental Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia; Corresponding author.
The current study report a convenient, simple, and low cost approach for the biogenic synthesis of CuO/Fe3O4 nanocomposites (NCs) from pumpkin seeds extract and their vitro cytotoxicity. The characterization of finally obtained CuO/Fe3O4 nanocomposites (NCs) performed using UV–Visible, FT-IR, XRD, XPS, GC-MS, SEM-EDX and TEM analysis. The formation and elemental analysis were determined using the energy-dispersive X-ray (EDX) microanalysis technique. The formation of rod-like monoclinic and spherical, having size range 5 nm–20 nm confirmed by scanning electron microscope (SEM) and transmission electron microscopy (TEM) respectively. Finally, the MTT assay of the synthesized composites was evaluated for toxicity against cancerous cell lines HCT-116 (Colon cancer cell) and A549 (human lung adenocarcinoma cell). The synthesized composite material showed moderate (IC50 = 199 μg/mL) to low (IC50 = 445 μg/mL) activity against HCT-116 and A549 cell lines, respectively.
