Foods (Aug 2024)

Structural Analysis and Antioxidant Activity of Alkaline-Extracted Glucans from <i>Hericium erinaceus</i>

  • Zhonghui Qiao,
  • Xiushi Jia,
  • Yuanning Wang,
  • Yuan Wang,
  • Yifa Zhou,
  • Fan Li,
  • Yunhe Qu,
  • Hairong Cheng

DOI
https://doi.org/10.3390/foods13172742
Journal volume & issue
Vol. 13, no. 17
p. 2742

Abstract

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An alkali-soluble β-glucan (AHEP-A-b, 20 kDa) purified from Hericium erinaceus fruiting bodies, was structurally characterized and examined for antioxidant activity. Methylation analysis and NMR spectroscopy show that the backbone of AHEP-A-b is composed of (1→6)-linked-D-β-glucopyran residues, branched at O-3 of glucopyranose (Glcp) residues with [→3)-β-D-Glcp-(1→] oligosaccharides or single unit of β-Glcp. Periodate oxidation analysis and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) indicate that the degree of polymerization (DP) of [→3)-β-D-Glcp-(1→] side chains is 2 to 8. Functionally, AHEP-A-b is a relatively strong antioxidant as demonstrated by using 2, 2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical (ABTS·+), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and hydroxyl radicals scavenging assays. The present study lays the foundation for further studies into structure-activity relationships of polysaccharides from H. erinaceus.

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