Drug Design, Development and Therapy (Mar 2017)

Does remifentanil attenuate renal ischemia–reperfusion injury better than dexmedetomidine in rat kidney?

  • Erkılıç E,
  • Kesimci E,
  • Alaybeyoğlu F,
  • Kılınç I,
  • Tural R,
  • Yazgan A,
  • Gümüş T,
  • Sepici Dinçel A,
  • Dumlu EG,
  • Kanbak O

Journal volume & issue
Vol. Volume11
pp. 677 – 683

Abstract

Read online

E Erkılıç,1 E Kesimci,1 F Alaybeyoğlu,1 I Kılınç,2 R Tural,3 A Yazgan,4 T Gümüş,1 A Sepici Dinçel,3 EG Dumlu,5 O Kanbak1 1Anesthesiology and Reanimation Department, 2General Surgery Department, Atatürk Training and Research Hospital, 3Biochemistry Department, Faculty of Medicine, Gazi University, 4Pathology Department, Atatürk Training and Research Hospital, 5General Surgery Clinic, Faculty of Medicine, Yıldırım Beyazit University, Ankara, Turkey Background: Ischemia–reperfusion (I/R) injury is a common cause of patient morbidity and mortality in the perioperative period. Patients undergoing long-lasting, abdominal, and urogenital surgeries with risk factors such as advanced age, peripheral artery disease, diabetes mellitus, renovascular disease, and congestive heart failure are candidates for acute kidney injury (AKI) due to impaired renal perfusion and decreased functional renal reserve. Pharmacological agents with multiple functions and anti-oxidative and anti-inflammation properties may be promising preventative strategies for AKI. Recently, dexmedetomidine (dex) has been postulated to have renoprotective effects.Objectives: We aimed to investigate the protective effects of an intravenous anesthetic remifentanil in renal I/R injury in the rat in comparison with dex. Materials and methods: A total of 30 Sprague Dawley adult rats were randomly assigned into five groups: the control group (group C, n=6), the sham group (group Sh, n=6, saline-infused rats without I/R injury), the saline group (group S, n=6, saline-infused rats with I/R injury), the remifentanil-treated group (group REM, n=6), and the dexmedetomidine-treated group (group DEX, n=6). The infusions (saline, remifentanil, and dex) were started after anesthesia induction and right nephrectomy and continued until the end of the surgical procedure. In I/R injury groups, the left renal artery and vein were occluded together by a clamp for 30 minutes and reperfusion lasted for 30 minutes. The rats were sacrificed after reperfusion, and the left kidney tissue was harvested. Blood samples were drawn from all animals to evaluate plasma neutrophil gelatinase-associated lipocalin (NGAL) at the beginning, 15 minutes after ischemia, 15 minutes after reperfusion, and 6 hours after the surgical procedure (T0, T1, T2, and T3, respectively). Results: The plasma NGAL levels exhibited increase at T1, T2, and T3 compared to the levels at T0 in group S (P<0.05). In group REM, there was a significant increase in plasma NGAL levels at T3 in comparison to those at T0, T1, and T2. The plasma NGAL levels at T2 in group S were significantly higher than those at T2 in group DEX (P<0.05). The groups S and REM showed significantly higher plasma NGAL levels at T3 compared to those at T0 (P<0.05). Upon histological examination, there was no difference among the study groups when left kidneys were evaluated (P>0.05). Conclusion: The NGAL levels and histopathological findings reflected protection by dex against renal I/R injury. However, the same exact results could not be mentioned for remifentanil depending on our study results. Keywords: ischemia–reperfusion, dexmedetomidine, remifentanil, neutrophil gelatinase-associated lipocalin

Keywords