Protective effects of sodium humate and its zinc and selenium chelate on the oxidative stress, inflammatory, and intestinal barrier damage of Salmonella Typhimurium-challenged broiler chickens

Yuying Fan; Wenzhu Zhou; Guili Li; Xuesong Liu; Peng Zhong; Kexin Liu; Yun Liu; Dong Wang

Poultry Science (May 2024)

Protective effects of sodium humate and its zinc and selenium chelate on the oxidative stress, inflammatory, and intestinal barrier damage of Salmonella Typhimurium-challenged broiler chickens

Yuying Fan,
Wenzhu Zhou,
Guili Li,
Xuesong Liu,
Peng Zhong,
Kexin Liu,
Yun Liu,
Dong Wang

Affiliations

Yuying Fan: Department of Veterinary Clinic, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China; Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Harbin, Heilongjiang, China
Wenzhu Zhou: Department of Veterinary Clinic, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China; Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Harbin, Heilongjiang, China
Guili Li: Qiqihar Center for Disease Control and Prevention Qiqihar, China
Xuesong Liu: Laboratory of Veterinary Pharmacology, Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar, China
Peng Zhong: Laboratory of Veterinary Pharmacology, Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar, China
Kexin Liu: Department of Veterinary Clinic, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China; Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Harbin, Heilongjiang, China
Yun Liu: Department of Veterinary Clinic, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China; Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Harbin, Heilongjiang, China; Corresponding author:
Dong Wang: Department of Veterinary Clinic, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China; Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Harbin, Heilongjiang, China; College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China

Journal volume & issue: Vol. 103, no. 5
p. 103541

Abstract

Read online

ABSTRACT: The objective of this study was to investigate the protective effects and mechanisms of dietary administration of sodium humate (HNa) and its zinc and selenium chelate (Zn/Se-HNa) in mitigating Salmonella Typhimurium (S. Typhi) induced intestinal injury in broiler chickens. Following the gavage of 109 CFU S. Typhi to 240 broilers from 21-d to 23-d aged, various growth performance parameters such as body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), and feed ratio (FCR) were measured before and after infection. Intestinal morphology was assessed to determine the villus height, crypt depth, and chorionic cryptologic ratio. To evaluate intestinal barrier integrity, levels of serum diamine oxidase (DAO), D-lactic acid, tight junction proteins, and the related genes were measured in each group of broilers. An analysis was conducted on inflammatory-related cytokines, oxidase activity, and Nuclear Factor Kappa B (NF-κB) and Nuclear factor erythroid2-related factor 2 (Nrf2) pathway-related proteins and mRNA expression. The results revealed a significant decrease in BW, ADG, and FCR in S. typhi-infected broilers. HNa tended to increase FCR (P = 0.056) while the supplementation of Zn/Se-HNa significantly restored BW and ADG (P < 0.05). HNa and Zn/Se-HNa exhibit favorable and comparable effects in enhancing the levels of serum DAO, D-lactate, and mRNA and protein expression of jejunum and ileal tight junction. In comparison to HNa, Zn/Se-HNa demonstrates a greater reduction in S. Typhi shedding in feces, as well as superior efficacy in enhancing the intestinal morphology, increasing serum catalase (CAT) activity, inhibiting pro-inflammatory cytokines, and suppressing the activation of the NF-κB pathway. Collectively, Zn/Se-HNa was a more effective treatment than HNa to alleviate adverse impact of S. Typhi infection in broiler chickens.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

About the journal

Abstract

Keywords