Медицинская иммунология (Oct 2017)
EXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA
Abstract
The objective of our study was to evaluate production of matrix metalloproteinase–2 and -9 in lacrimal fluid, as well as СFH gene polymorphism in a group of patients with primary open-angle glaucoma. The study was performed with 120 eyes of patients with primary open-angle glaucoma (POAG) including 34 eyes from the first-stage disease; 35 eyes, from the 2nd stage; 35, at the 3rd stage of the disorder, and 16 eyes with stage 4 POAG. Control group consisted of 25 glaucoma-free patients. Tear fluid served as a material for biochemical tests. The levels of MMP-2 and MMP-9 were determined by sandwich ELISA for the both eyes. Venous blood was used for genetic studies. Genomic human DNA was isolated from the whole blood leukocytes, a polymerase chain reaction (PCR) was performed. The results of this study were analyzed using a package of SAS applied statistical programs (Statistical Analysis System, SAS Institute Inc., USA) employing standard algorithms of variation statistics, i.e., correlation analysis, contingency tables, and various types of intergroup comparisons for the parameters studied. The following results were obtained: average indices for metalloproteinases-2 and -9 were found to be increased upon progression of the disease. Correlation analysis of contingency relationships was performed for morphometric, clinical, functional parameters, and quantitative indices of MMP-2 and MMP-9. We also studied prevalence of CFH gene polymorphismic alleles depending on the stage of glaucoma development. One may conclude that our study provided evidence for a relationship between MMP-2 and MMP-9 production, and СFH gene polymorphism, thus expanding a list of host genetic mutations which could be potentially involved into pathogenesis of primary open-angle glaucoma.
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