Chromatin Remodeling BAF155 Subunit Regulates the Genesis of Basal Progenitors in Developing Cortex
Ramanathan Narayanan,
Linh Pham,
Cemil Kerimoglu,
Takashi Watanabe,
Ricardo Castro Hernandez,
Godwin Sokpor,
Pauline Antonie Ulmke,
Kamila A. Kiszka,
Anton B. Tonchev,
Joachim Rosenbusch,
Rho H. Seong,
Ulrike Teichmann,
Jens Frahm,
Andre Fischer,
Stefan Bonn,
Anastassia Stoykova,
Jochen F. Staiger,
Tran Tuoc
Affiliations
Ramanathan Narayanan
Institute of Neuroanatomy, University Medical Center, Georg-August-University, 37075 Goettingen, Germany
Linh Pham
Institute of Neuroanatomy, University Medical Center, Georg-August-University, 37075 Goettingen, Germany
Cemil Kerimoglu
German Center for Neurodegenerative Diseases, 37075 Goettingen, Germany
Takashi Watanabe
Max-Planck-Institute for Biophysical Chemistry, 37077 Goettingen, Germany
Ricardo Castro Hernandez
Institute of Neuroanatomy, University Medical Center, Georg-August-University, 37075 Goettingen, Germany
Godwin Sokpor
Institute of Neuroanatomy, University Medical Center, Georg-August-University, 37075 Goettingen, Germany
Pauline Antonie Ulmke
Institute of Neuroanatomy, University Medical Center, Georg-August-University, 37075 Goettingen, Germany
Kamila A. Kiszka
Institute of Neuroanatomy, University Medical Center, Georg-August-University, 37075 Goettingen, Germany; DFG, Center for Molecular Physiology of the Brain (CMPB), 37075 Goettingen, Germany
Anton B. Tonchev
Max-Planck-Institute for Biophysical Chemistry, 37077 Goettingen, Germany; Department of Anatomy and Cell Biology, Medical University-Varna, BG-9002 Varna, Bulgaria
Joachim Rosenbusch
Institute of Neuroanatomy, University Medical Center, Georg-August-University, 37075 Goettingen, Germany
Rho H. Seong
Department of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 08826, Korea
Ulrike Teichmann
Max-Planck-Institute for Biophysical Chemistry, 37077 Goettingen, Germany
Jens Frahm
Max-Planck-Institute for Biophysical Chemistry, 37077 Goettingen, Germany
Andre Fischer
German Center for Neurodegenerative Diseases, 37075 Goettingen, Germany; DFG, Center for Molecular Physiology of the Brain (CMPB), 37075 Goettingen, Germany
Stefan Bonn
German Center for Neurodegenerative Diseases, 37075 Goettingen, Germany
Anastassia Stoykova
Max-Planck-Institute for Biophysical Chemistry, 37077 Goettingen, Germany; DFG, Center for Molecular Physiology of the Brain (CMPB), 37075 Goettingen, Germany; Corresponding author
Jochen F. Staiger
Institute of Neuroanatomy, University Medical Center, Georg-August-University, 37075 Goettingen, Germany; DFG, Center for Molecular Physiology of the Brain (CMPB), 37075 Goettingen, Germany
Tran Tuoc
Institute of Neuroanatomy, University Medical Center, Georg-August-University, 37075 Goettingen, Germany; DFG, Center for Molecular Physiology of the Brain (CMPB), 37075 Goettingen, Germany; Corresponding author
Summary: The abundance of basal progenitors (BPs), basal radial glia progenitors (bRGs) and basal intermediate progenitors (bIPs), in primate brain has been correlated to the high degree of cortical folding. Here we examined the role of BAF155, a subunit of the chromatin remodeling BAF complex, in generation of cortical progenitor heterogeneity. The conditional deletion of BAF155 led to diminished bIP pool and increased number of bRGs, due to delamination of apical RGs. We found that BAF155 is required for normal activity of neurogenic transcription factor PAX6, thus controlling the expression of genes that are involved in bIP specification, cell-cell interaction, and establishment of adherens junction. In a PAX6-dependent manner, BAF155 regulates the expression of the CDC42 effector protein CEP4, thereby controlling progenitor delamination. Furthermore, BAF155-dependent chromatin remodeling seems to exert a specific role in the genesis of BPs through the regulation of human RG-specific genes (such as Foxn4) that possibly acquired evolutionary significance. : Neuroscience; Molecular Neuroscience; Developmental Neuroscience Subject Areas: Neuroscience, Molecular Neuroscience, Developmental Neuroscience
