Nature Communications (Jan 2020)

Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

  • Jinjin Shen,
  • Xiaoming Zhou,
  • Yuanyue Shan,
  • Huahua Yue,
  • Ru Huang,
  • Jiaming Hu,
  • Da Xing

DOI
https://doi.org/10.1038/s41467-019-14135-9
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 10

Abstract

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The detection of pathogens in food and clinical samples remains a challenge. Here, Shen et al. present a detection system, involving a combination of nucleic acid-based allosteric probes and CRISPR-Cas13a components, that can detect very low numbers of a bacterial pathogen in milk and serum samples without isolation.