Validation, optimisation, and application data in support of the development of a targeted selected ion monitoring assay for degraded cardiac troponin T
Alexander S. Streng,
Douwe de Boer,
Freek G. Bouwman,
Edwin C.M. Mariman,
Arjen Scholten,
Marja P. van Dieijen-Visser,
Will K.W.H. Wodzig
Affiliations
Alexander S. Streng
Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, the Netherlands
Douwe de Boer
Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, the Netherlands
Freek G. Bouwman
Department of Human Biology, Maastricht University, Maastricht, the Netherlands
Edwin C.M. Mariman
Department of Human Biology, Maastricht University, Maastricht, the Netherlands
Arjen Scholten
Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands; Netherlands Proteomics Centre, Utrecht University, Utrecht, the Netherlands; Current working address: Janssen, Pharmaceutical Companies of Johnson&Johnson, Infectious Diseases and Vaccines, Leiden, the Netherlands
Marja P. van Dieijen-Visser
Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, the Netherlands
Will K.W.H. Wodzig
Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, the Netherlands; Corresponding author at: Central Diagnostic Laboratory, Maastricht University Medical Centre, P. Debyelaan 25. P.O. Box 5800, 6202 AZ Maastricht, the Netherlands. Tel.: +31 043 3874977; fax: +31 043 3874692.
Cardiac troponin T (cTnT) fragmentation in human serum was investigated using a newly developed targeted selected ion monitoring assay, as described in the accompanying article: “Development of a targeted selected ion monitoring assay for the elucidation of protease induced structural changes in cardiac troponin T” [1]. This article presents data describing aspects of the validation and optimisation of this assay. The data consists of several figures, an excel file containing the results of a sequence identity search, and a description of the raw mass spectrometry (MS) data files, deposited in the ProteomeXchange repository with id PRIDE: PXD003187.
