The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system

Tao Dong; Siyao Zhang; Yun Chang; Rui Bai; Youxu Jiang; Shuhong Ma; Ya'nan Li; Hong Feng Jiang; Wen-Jing Lu

Stem Cell Research (Mar 2021)

The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system

Tao Dong,
Siyao Zhang,
Yun Chang,
Rui Bai,
Youxu Jiang,
Shuhong Ma,
Ya'nan Li,
Hong Feng Jiang,
Wen-Jing Lu

Affiliations

Tao Dong: Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling‐Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China
Siyao Zhang: Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling‐Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China
Yun Chang: Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling‐Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China
Rui Bai: Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling‐Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China
Youxu Jiang: Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling‐Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China
Shuhong Ma: Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling‐Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China
Ya'nan Li: Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling‐Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China
Hong Feng Jiang: Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling‐Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China; Corresponding authors at: Research Institute Building, Room 323, Beijing Anzhen Hospital, 2 Anzhen Road, Chaoyang District, Beijing 100029, China.
Wen-Jing Lu: Beijing Laboratory for Cardiovascular Precision Medicine, The Key Laboratory of Remodeling‐Related Cardiovascular Disease, Ministry of Education, Beijing Collaborative Innovation Center for Cardiovascular Disorders, Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China; Corresponding authors at: Research Institute Building, Room 323, Beijing Anzhen Hospital, 2 Anzhen Road, Chaoyang District, Beijing 100029, China.

Journal volume & issue: Vol. 51
p. 102196

Abstract

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SNTA1 encodes α1-syntrophin, a scaffold protein, which is a component of the dystrophin-associated protein complex. Additionally, α1-syntrophin interacts with SCN5A and nNOS–PMCA4b complex in cardiomyocytes. SNTA1 is a susceptibility locus for arrhythmia and cardiomyopathy. We generated a homozygous SNTA1 knockout human embryonic stem cell (H9SNTA1KO) using the CRISPR/Cas9 system. H9SNTA1KO maintained pluripotency and a normal karyotype and differentiated into three germ layers in vivo.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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