The Expermintal Infection of L.monocytogenes &Isolation it from Egg, Oviduct & Ovary

Abstract

The aim of the present study to detect the transmission of L.monocytogenes from experimental infected chickens to Egg products ,Ovary,Oviduct .&histopathological changes in these organs. 2groups of chickn ,1st group(12 animals)infected with L.monocytogenes 1ml/chick.SC,(1×1010 cfu/ml),2nd group injected 1ml/chick normal salin as control.eggs were collected for isolate Listeria ,animal killed in (7,15,24,30,34,39,42)days,for isolateListeria from ovary,oviduct&samples taken for histopathological examination. The results show theres no characterstic signs in infected chickens except depression,anoxia&and theres marked decrease of egg production, qualititativly & quantitativly. L.monocytogenes isolated from egg ( shell, ,yolk,albumine)&persiste to (31,28&19) dayses respectively,also Listeria isolate from ovary&oviduct in chickens which killed in (7,15,24,30)day,¬ isolate from chickens that killed in day (39,34,42)or in control. The histopathological changes theres congestion of the organs ,diffuse infiltration of infilammatory cells such as lymphocyte , neutrophils & macrophage. also theres odeama, multi necrosis foci &desqumation.