Molecular Plant-Microbe Interactions (Nov 2003)

Isolation of a Polygalacturonase-Inhibiting Protein (PGIP) from Wheat

  • Gabré Kemp,
  • Carl W. Bergmann,
  • Ron Clay,
  • Amie J. Van der Westhuizen,
  • Zacharias A. Pretorius

DOI
https://doi.org/10.1094/MPMI.2003.16.11.955
Journal volume & issue
Vol. 16, no. 11
pp. 955 – 961

Abstract

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Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP.