Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
Wireeya Chawjiraphan,
Chayachon Apiwat,
Khoonsake Segkhoonthod,
Kiatnida Treerattrakoon,
Preedee Pinpradup,
Nuankanya Sathirapongsasuti,
Prapasiri Pongprayoon,
Patraporn Luksirikul,
Patcharee Isarankura-Na-Ayudhya,
Deanpen Japrung
Affiliations
Wireeya Chawjiraphan
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand
Chayachon Apiwat
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand; Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand
Khoonsake Segkhoonthod
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand
Kiatnida Treerattrakoon
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand; Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow, United Kingdom
Preedee Pinpradup
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand
Nuankanya Sathirapongsasuti
Section of Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Prapasiri Pongprayoon
Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand; Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand.
Patraporn Luksirikul
Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand
Patcharee Isarankura-Na-Ayudhya
Department of Medical Technology, Faculty of Allied Health Science, Thammasat University, Pathumthani, Thailand
Deanpen Japrung
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand; Corresponding authors.
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1
