Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine

Kezhuo Zhang; Huadong Cai; Min Lu; Daixian Wei; Jiaqi Yin; Nengshui Ding; Weihua Lai; Juan Peng

doi:10.1080/09540105.2022.2080188

Food and Agricultural Immunology (Dec 2022)

Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine

Kezhuo Zhang,
Huadong Cai,
Min Lu,
Daixian Wei,
Jiaqi Yin,
Nengshui Ding,
Weihua Lai,
Juan Peng

Affiliations

Kezhuo Zhang: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Huadong Cai: Ganzhou City Animal Husbandry Development & Animal Epidemic Diseases Prevention and Control Center, Ganzhou, People’s Republic of China
Min Lu: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Daixian Wei: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Jiaqi Yin: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Nengshui Ding: State Key Laboratory of Food Safety Technology for Meat Products, Xiamen, People’s Republic of China
Weihua Lai: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Juan Peng: School of Food Science, Nanchang University, Nanchang, People’s Republic of China

DOI: https://doi.org/10.1080/09540105.2022.2080188
Journal volume & issue: Vol. 33, no. 1
pp. 403 – 418

Abstract

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A bispecific monoclonal antibody (BsMAb) that can simultaneously recognize aflatoxin B1 (AFB1) and amantadine (AMD) was prepared. Quantum dot nanobead immunochromatographic assay (QB-ICA) based on the BsMAb was developed for the simultaneous detection of AFB1 and AMD in four feed samples (suckling pig feed, piglet feed, sow feed, and compound feed for laying ducks). Under optimal experimental conditions, the LOD values of QB-ICA for AFB1 in four feed samples were 0.188, 0.221, 0.233, and 0.306 μg/kg, respectively. The LOD values of QB-ICA for AMD in the same feed samples were 0.125, 0.187, 0.076, and 0.191 μg/kg, respectively. The recovery rates of QB-ICA for AFB1 and AMD ranged from 91.55% to 150.87% and from 91.62% to 138.42%, respectively. The variation coefficients were all less than 15%. The proposed QB-ICA had high reliability and specificity for the simultaneous detection of AFB1 and AMD, providing a platform for simultaneous detection of hazardous substances.

Published in Food and Agricultural Immunology

ISSN: 0954-0105 (Print); 1465-3443 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Agriculture: Agriculture (General); Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: https://www.tandfonline.com/journals/cfai

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