Gamma-glutamyl transpeptidase activity in transformed cells upon action on epidermal growth factor receptor

Abstract

Researched gamma-glutamintranspeptydase activity (GGT activity) of HeLa cell line (human cervical cancer) and Hep G2 (human hepatocarcinoma) the influence on epidermal growth factor by mitogen and monoclonal antibodies to this receptor – herceptin. Background levels of GGT activity in Hep G2 cells lines prevailed almost 10 times (p <0,01) comparing with HeLa cells and was 1,95 ± 0,2 nkat/ml. Was discovered that influence of EGF and herceptinon HeLa cells doesn't cause significant differences, while EGF that effected hepatocarcinomas cells increase GGT activityon 20%. Herceptin, conversely inhibite twice (p<0,05) comparing with controls.The observed difference GGT activity in cell lines HeLa and Hep G2 shows the selective expression of this enzyme in Hep G2 cells and can be used as a marker of malignancy transformed cells of hepatocellular origin.

Keywords

• gamma-glutamintranspeptidase, herceptin, EGF