Investigating the Neurotoxic Impacts of Arsenic and the Neuroprotective Effects of Dictyophora Polysaccharide Using SWATH-MS-Based Proteomics
Jun Zhang,
Ting Hu,
Yi Wang,
Xinglai Zhang,
Huajie Zhang,
Jing Lin,
Xiaoxiao Tang,
Xukun Liu,
Margy Chen,
Naseer Ullah Khan,
Liming Shen,
Peng Luo
Affiliations
Jun Zhang
The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550025, China
Ting Hu
The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550025, China
Yi Wang
The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550025, China
Xinglai Zhang
The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550025, China
Huajie Zhang
College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, China
Jing Lin
College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, China
Xiaoxiao Tang
College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, China
Xukun Liu
College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, China
Margy Chen
Department of Psychology, Emory University, Atlanta, GA 30322, USA
Naseer Ullah Khan
College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, China
Liming Shen
College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, China
Peng Luo
The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550025, China
Arsenic (As) is one of the most important toxic elements in the natural environment. Currently, although the assessment of the potential health risks of chronic arsenic poisoning has received great attention, the research on the effects of arsenic on the brain is still limited. It has been reported that dictyophora polysaccharide (DIP), a common bioactive natural compound found in dietary plants, could reduce arsenic toxicity. Following behavioral research, comparative proteomics was performed to explore the molecular mechanism of arsenic toxicity to the hippocampi of SD (Sprague Dawley) rats and the protective effect of DIP. The results showed that exposure to arsenic impaired the spatial learning and memory ability of SD rats, while DIP treatment improved both the arsenic-exposed rats. Proteomic analysis showed that arsenic exposure dysregulated the expression of energy metabolism, apoptosis, synapse, neuron, and mitochondria related proteins in the hippocampi of arsenic-exposed rats. However, DIP treatment reversed or restored the expression levels of these proteins, thereby improving the spatial learning and memory ability of arsenic-exposed rats. This study is the first to use high-throughput proteomics to reveal the mechanism of arsenic neurotoxicity in rats as well as the protective mechanism of DIP against arsenic neurotoxicity.
