Frontiers in Microbiology (Jun 2025)
Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v
- Xiong-nan Chen,
- Xiong-nan Chen,
- Xiong-nan Chen,
- Gao-xi Zhu,
- Gao-xi Zhu,
- Gao-xi Zhu,
- Yi-xian Li,
- Yi-han Hao,
- Yi-han Hao,
- Yi-han Hao,
- Yi-fan Liang,
- Yi-fan Liang,
- Chen Hu,
- Ying-shuo Sun,
- Ying-shuo Sun,
- Yun-zhao Peng,
- Yun-zhao Peng,
- Xi Li,
- Xi Li,
- Xi Li,
- Zhao Huang,
- Zhao Huang,
- Zhao Huang,
- Gui-hong Zhang,
- Gui-hong Zhang,
- Gui-hong Zhang,
- Lang Gong,
- Lang Gong,
- Lang Gong,
- Ze-zhong Zheng,
- Ze-zhong Zheng,
- Ze-zhong Zheng,
- Ze-zhong Zheng
Affiliations
- Xiong-nan Chen
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Xiong-nan Chen
- Research Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, China
- Xiong-nan Chen
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Gao-xi Zhu
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Gao-xi Zhu
- Research Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, China
- Gao-xi Zhu
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Yi-xian Li
- School of Medicine, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China
- Yi-han Hao
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yi-han Hao
- Research Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, China
- Yi-han Hao
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Yi-fan Liang
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yi-fan Liang
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Chen Hu
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Ying-shuo Sun
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Ying-shuo Sun
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Yun-zhao Peng
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yun-zhao Peng
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Xi Li
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Xi Li
- Research Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, China
- Xi Li
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Zhao Huang
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Zhao Huang
- Research Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, China
- Zhao Huang
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Gui-hong Zhang
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Gui-hong Zhang
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Gui-hong Zhang
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Lang Gong
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Lang Gong
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Lang Gong
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Ze-zhong Zheng
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Ze-zhong Zheng
- Research Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, China
- Ze-zhong Zheng
- Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, China
- Ze-zhong Zheng
- Wen’s Food Group, Yunfu, China
- DOI
- https://doi.org/10.3389/fmicb.2025.1585335
- Journal volume & issue
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Vol. 16
Abstract
BackgroundThe African swine fever virus (ASFV) is a highly pathogenic double-stranded DNA virus that poses a significant threat to the global swine industry. Although the CD2v protein encoded by ASFV is a key factor in viral immune evasion and pathogenicity, the mechanism underlying its interaction with host proteins remains unclear.MethodsThe aim of this study was to identify a range of potential host proteins that interact with the CD2v protein using the membrane yeast two-hybrid technology.ResultsThrough subsequent validation experiments and functional analyses, we discovered that these proteins are involved in critical cellular processes such as translational regulation, inflammatory responses, immune signaling, and iron metabolism. Furthermore, interaction network and functional enrichment analyses revealed that ASFV might influence host cell functions through multiple pathways to facilitate viral replication.ConclusionThis study provides new insights into the pathogenic mechanisms of ASFV and offers valuable clues for identifying antiviral targets.
Keywords
- African swine fever virus
- CD2v
- dual membrane system
- membrane protein interaction
- interaction network
