RETRACTED ARTICLE: Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells

Ruifeng Zhao; Qisheng Zuo; Xia Yuan; Kai Jin; Jing Jin; Ying Ding; Chen Zhang; Tingting Li; Jingyi Jiang; Jiancheng Li; Ming Zhang; Xiang Shi; Hongyan Sun; Yani Zhang; Qi Xu; Guobin Chang; Zhenhua Zhao; Bing Li; Xinsheng Wu; Yang Zhang; Jiuzhou Song; Guohong Chen; Bichun Li

doi:10.1038/s41467-021-23242-5

Nature Communications (May 2021)

RETRACTED ARTICLE: Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells

Ruifeng Zhao,
Qisheng Zuo,
Xia Yuan,
Kai Jin,
Jing Jin,
Ying Ding,
Chen Zhang,
Tingting Li,
Jingyi Jiang,
Jiancheng Li,
Ming Zhang,
Xiang Shi,
Hongyan Sun,
Yani Zhang,
Qi Xu,
Guobin Chang,
Zhenhua Zhao,
Bing Li,
Xinsheng Wu,
Yang Zhang,
Jiuzhou Song,
Guohong Chen,
Bichun Li

Affiliations

Ruifeng Zhao: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Qisheng Zuo: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Xia Yuan: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Kai Jin: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Jing Jin: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Ying Ding: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Chen Zhang: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Tingting Li: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Jingyi Jiang: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Jiancheng Li: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Ming Zhang: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Xiang Shi: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Hongyan Sun: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Yani Zhang: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Qi Xu: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Guobin Chang: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Zhenhua Zhao: The Poultry Research Institute of Chinese Academy of Agricultural Sciences
Bing Li: The Poultry Research Institute of Chinese Academy of Agricultural Sciences
Xinsheng Wu: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Yang Zhang: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Jiuzhou Song: Department of Animal & Avian Sciences, University of Maryland
Guohong Chen: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University
Bichun Li: Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University

DOI: https://doi.org/10.1038/s41467-021-23242-5
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 13

Abstract

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Abstract The allogeneic transplantation of primordial germ cells (PGCs) derived from somatic cells overcomes the limitation of avian cloning. Here, we transdifferentiate chicken embryo fibroblasts (CEFs) from black feathered Langshan chickens to PGCs and transplant them into White Plymouth Rock chicken embryos to produce viable offspring with characteristics inherited from the donor. We express Oct4/Sox2/Nanog/Lin28A (OSNL) to reprogram CEFs to induced pluripotent stem cells (iPSCs), which are further induced to differentiate into PGCs by BMP4/BMP8b/EGF. DNA demethylation, histone acetylation and glycolytic activation elevate the iPSC induction efficiency, while histone acetylation and glycolytic inhibition facilitate PGCs formation. The induced PGCs (iPGCs) are transplanted into the recipients, which are self-crossed to produce 189/509 somatic cells derived chicken with the donor’s characteristics. Microsatellite analysis and genome sequencing confirm the inheritance of genetic information from the donor. Thus, we demonstrate the feasibility of avian cloning from somatic cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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