Can Storage Stability and Simulated Gastrointestinal Behavior Change the Cytotoxic Effects of Concentrated Guava Leaves Extract against Human Lung Cancer Cells?
Giordana Demaman Arend,
Silvani Verruck,
Naira Fernanda Zanchett Schneider,
Cláudia Maria Oliveira Simões,
Marcus Vinícius Tres,
Elane Schwinden Prudêncio,
José Carlos Cunha Petrus,
Katia Rezzadori
Affiliations
Giordana Demaman Arend
Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil
Silvani Verruck
Department of Food Science and Technology, Federal University of Santa Catarina, Av. Ademar Gonzaga, 1346, Itacorubi, Florianópolis 88034-000, SC, Brazil
Naira Fernanda Zanchett Schneider
Laboratory of Applied Virology, Department of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianópolis 88035-972, SC, Brazil
Cláudia Maria Oliveira Simões
Laboratory of Applied Virology, Department of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianópolis 88035-972, SC, Brazil
Marcus Vinícius Tres
Laboratory of Agroindustrial Processes Engineering—LAPE, Federal University of Santa Maria, Cachoeira do Sul 96503-205, RS, Brazil
Elane Schwinden Prudêncio
Department of Food Science and Technology, Federal University of Santa Catarina, Av. Ademar Gonzaga, 1346, Itacorubi, Florianópolis 88034-000, SC, Brazil
José Carlos Cunha Petrus
Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil
Katia Rezzadori
Department of Food Science and Technology, Federal University of Santa Catarina, Av. Ademar Gonzaga, 1346, Itacorubi, Florianópolis 88034-000, SC, Brazil
The influence of storage stability and simulated gastrointestinal behavior of different extracts of guava leaves extracts (NC: not concentrated, and C10 and C20: concentrated by nanofiltration) was evaluated based on their total phenolic compound (TPC) contents and antioxidant activity as well as on their cytotoxic effects on A549 and Vero cells. The results showed that C10 and C20 presented high stability for 125 days probably due to their high TPC contents and antioxidant activity. The simulated gastrointestinal behavior modified their TPC contents; however, after all digestion steps, the TPC values were higher than 70%, which means that they were still available to exert their bioactivities. Additionally, the cytotoxic effects of these extracts were evaluated before and after the simulated gastrointestinal behavior or under different storage conditions. C10 presented the best selectivity indices (SI) values (IC50 Vero cells/IC50 A549 cells) at both conditions suggesting that it can be considered a potential extract to be developed as a functional food due to its resistance to the gastrointestinal digestion and storage conditions tested.
