Journal of Pharmacological Sciences (Jan 2013)

Protective Effect of Luteolin on an Oxidative-Stress Model Induced by Microinjection of Sodium Nitroprusside in Mice

  • Qand Agha Nazari,
  • Toshiaki Kume,
  • Yuki Takada-Takatori,
  • Yasuhiko Izumi,
  • Akinori Akaike

DOI
https://doi.org/10.1254/jphs.13019fp
Journal volume & issue
Vol. 122, no. 2
pp. 109 – 117

Abstract

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Accumulating lines of evidence showed that luteolin, a polyphenolic compound, has potent neuroprotective effects. The purpose of this study was to examine whether luteolin can protect against sodium nitroprusside (SNP)-induced oxidative damage in mouse brain. Intrastriatal co-injection of luteolin (3 – 30 nmol) with SNP (10 nmol) dose-dependently protected against brain damage and motor dysfunction. Oral administrations of luteolin (600 – 1200 mg/kg) dose-dependently protected against brain damage and motor dysfunction induced by striatal injection of SNP. Furthermore, luteolin (30 – 100 μM) concentration dependently protected against Fe2+-induced lipid peroxidation in mouse brain homogenate. Luteolin (1 – 100 μg/ml) showed potent DPPH radical scavenging ability, when compared with ascorbic acid and glutathione. Finally, a ferrozine assay showed that luteolin (30 – 100 μg/ml) has Fe2+-chelating ability, but this was weaker than that of ethylenediaminetetraacetic acid. These results suggest that intrastriatal or oral administration of luteolin protected mice brain from SNP-induced oxidative damage by scavenging and chelating effects. Keywords:: sodium nitroprusside, brain damage, motor dysfunction, oxidative stress, scavenging