Seroprevalence study of bubaline brucellosis in Kota Division of Rajasthan using various serodiagnostic tests

Abstract

The study was undertaken to investigate the seroprevalence of brucellosis in buffaloes of Sangod, Mandana, Bundi, Talera, Chhabra, Jhalrapatan and Dag regions of Kota division of Rajasthan. Blood samples (436) were collected for detection of Brucella antibody using Rose Bengal plate test (RBPT), standard tube agglutination test (STAT), heat inactivation test (HIT), 2-mercaptoethanol test (2-MET), agar gel immunodiffusion test (AGID) and avidin biotin enzyme linked immunosorobent assay (AB ELISA). Overall seroprevalence was recorded as 37.84% by AB ELISA, 35.09% by RBPT, 29.59% by STAT, 26.15% in HIT, 25.23% in 2-MET and 13.99% in AGID. AB ELISA was found as the most sensitive whereas AGID was the least sensitive test. Higher number of females (38.46%) showed seropositivity as compared to males (30.30%). The results of the study indicated (a) relative suitability of ELISA as a screening test, and (b) endemacity of infection in villages of Kota division, Rajasthan.

Keywords

• AB ELISA
• Brucellosis
• Buffaloes
• Serology