Hsp90aa1/JUN/Ccl2 regulatory axis mediates migration and differentiation of NSPCs, promoting the onset and progression of early post-ischemic stroke epilepsy

Shuntong Hu; Yongzhong Tang; Xiaobo Li; Wenjun Li; Yini Zeng; Mi Jiang; Ru Chen; Ping Zheng; Liang Yang; Zhi Song; Dujie Xie; Yiwei Chen; Yi Yuan

Neurobiology of Disease (Oct 2024)

Hsp90aa1/JUN/Ccl2 regulatory axis mediates migration and differentiation of NSPCs, promoting the onset and progression of early post-ischemic stroke epilepsy

Shuntong Hu,
Yongzhong Tang,
Xiaobo Li,
Wenjun Li,
Yini Zeng,
Mi Jiang,
Ru Chen,
Ping Zheng,
Liang Yang,
Zhi Song,
Dujie Xie,
Yiwei Chen,
Yi Yuan

Affiliations

Shuntong Hu: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China
Yongzhong Tang: Department of Anesthesiology, The Third Xiangya Hospital, Central South University, Changsha, China
Xiaobo Li: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China
Wenjun Li: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China
Yini Zeng: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China
Mi Jiang: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China; Department of Neurology, University of Michigan, Ann Arbor, MI, USA
Ru Chen: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China
Ping Zheng: Department of Neurosurgery, Shanghai Pudong New Area People's Hospital, Shanghai, China
Liang Yang: Department of Neurosurgery, The Third Xiangya Hospital, Central South University, Changsha, China
Zhi Song: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China
Dujie Xie: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China; Corresponding authors at: Department of Neurology, The Third Xiangya Hospital of Central South University, No. 138 Tongzipo Road, Yuelu District, Changsha, Hunan Province, China.
Yiwei Chen: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China; Corresponding authors at: Department of Neurology, The Third Xiangya Hospital of Central South University, No. 138 Tongzipo Road, Yuelu District, Changsha, Hunan Province, China.
Yi Yuan: Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China; Corresponding authors at: Department of Neurology, The Third Xiangya Hospital of Central South University, No. 138 Tongzipo Road, Yuelu District, Changsha, Hunan Province, China.

Journal volume & issue: Vol. 200
p. 106635

Abstract

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Early-onset epilepsy following ischemic stroke is a severe neurological condition, the pathogenesis of which remains incompletely understood. Recent studies suggest that Neural stem/progenitor cells (NSPCs) play a crucial role in the disease process, yet the precise molecular mechanisms regulating NSPCs have not been thoroughly investigated. This study utilized single-cell transcriptome sequencing and bioinformatics analysis to identify disease-related genes, which were subsequently validated in both in vitro and in vivo experiments. The findings revealed that Hsp90aa1 (heat shock protein 90 kDa alpha, class A member 1), Jun proto-oncogene (JUN), and CC Motif Ligation 2 (Ccl2) constitute an important regulatory axis influencing the migration and differentiation of NSPCs, potentially impacting the onset and progression of early-onset epilepsy post-ischemic stroke. Additionally, the expression of Hsp90aa1 was found to influence the likelihood of seizure occurrence and the severity of brain ischemia.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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