Nature Communications (Dec 2020)
Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
- Tomáš Kouba,
- Tomáš Koval’,
- Petra Sudzinová,
- Jiří Pospíšil,
- Barbora Brezovská,
- Jarmila Hnilicová,
- Hana Šanderová,
- Martina Janoušková,
- Michaela Šiková,
- Petr Halada,
- Michal Sýkora,
- Ivan Barvík,
- Jiří Nováček,
- Mária Trundová,
- Jarmila Dušková,
- Tereza Skálová,
- URee Chon,
- Katsuhiko S. Murakami,
- Jan Dohnálek,
- Libor Krásný
Affiliations
- Tomáš Kouba
- EMBL Grenoble, 71 Avenue des Martyrs
- Tomáš Koval’
- Institute of Biotechnology of the Czech Academy of Sciences, Centre BIOCEV, Průmyslová 595
- Petra Sudzinová
- Institute of Microbiology of The Czech Academy of Sciences
- Jiří Pospíšil
- Institute of Microbiology of The Czech Academy of Sciences
- Barbora Brezovská
- Institute of Microbiology of The Czech Academy of Sciences
- Jarmila Hnilicová
- Institute of Microbiology of The Czech Academy of Sciences
- Hana Šanderová
- Institute of Microbiology of The Czech Academy of Sciences
- Martina Janoušková
- Institute of Microbiology of The Czech Academy of Sciences
- Michaela Šiková
- Institute of Microbiology of The Czech Academy of Sciences
- Petr Halada
- Institute of Microbiology of The Czech Academy of Sciences
- Michal Sýkora
- Institute of Molecular Genetics of the Czech Academy of Sciences
- Ivan Barvík
- Faculty of Mathematics and Physics, Institute of Physics, Charles University
- Jiří Nováček
- CEITEC, Masaryk University
- Mária Trundová
- Institute of Biotechnology of the Czech Academy of Sciences, Centre BIOCEV, Průmyslová 595
- Jarmila Dušková
- Institute of Biotechnology of the Czech Academy of Sciences, Centre BIOCEV, Průmyslová 595
- Tereza Skálová
- Institute of Biotechnology of the Czech Academy of Sciences, Centre BIOCEV, Průmyslová 595
- URee Chon
- Department of Biochemistry and Molecular Biology, The Center for RNA Molecular Biology, Pennsylvania State University
- Katsuhiko S. Murakami
- Department of Biochemistry and Molecular Biology, The Center for RNA Molecular Biology, Pennsylvania State University
- Jan Dohnálek
- Institute of Biotechnology of the Czech Academy of Sciences, Centre BIOCEV, Průmyslová 595
- Libor Krásný
- Institute of Microbiology of The Czech Academy of Sciences
- DOI
- https://doi.org/10.1038/s41467-020-20158-4
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 13
Abstract
The bacterial helicase-like transcription factor HelD associates with the RNA polymerase (RNAP) and recycles stalled transcription complexes. Here, the authors present the cryo-EM structures of three Mycobacterium smegmatis HelD bound RNAP complexes and further show that HelD can prevent the binding of the RNAP core to non-specific DNA and also actively removes RNAP from stalled elongation complexes.
