eLife (Oct 2020)
Cytotoxic T cells swarm by homotypic chemokine signalling
- Jorge Luis Galeano Niño,
- Sophie V Pageon,
- Szun S Tay,
- Feyza Colakoglu,
- Daryan Kempe,
- Jack Hywood,
- Jessica K Mazalo,
- James Cremasco,
- Matt A Govendir,
- Laura F Dagley,
- Kenneth Hsu,
- Simone Rizzetto,
- Jerzy Zieba,
- Gregory Rice,
- Victoria Prior,
- Geraldine M O'Neill,
- Richard J Williams,
- David R Nisbet,
- Belinda Kramer,
- Andrew I Webb,
- Fabio Luciani,
- Mark N Read,
- Maté Biro
Affiliations
- Jorge Luis Galeano Niño
- EMBL Australia, Single Molecule Science node, University of New South Wales, Sydney, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- Sophie V Pageon
- ORCiD
- EMBL Australia, Single Molecule Science node, University of New South Wales, Sydney, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- Szun S Tay
- ORCiD
- EMBL Australia, Single Molecule Science node, University of New South Wales, Sydney, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- Feyza Colakoglu
- EMBL Australia, Single Molecule Science node, University of New South Wales, Sydney, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- Daryan Kempe
- EMBL Australia, Single Molecule Science node, University of New South Wales, Sydney, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- Jack Hywood
- Sydney Medical School, The University of Sydney, Sydney, Australia
- Jessica K Mazalo
- EMBL Australia, Single Molecule Science node, University of New South Wales, Sydney, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- James Cremasco
- EMBL Australia, Single Molecule Science node, University of New South Wales, Sydney, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- Matt A Govendir
- EMBL Australia, Single Molecule Science node, University of New South Wales, Sydney, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- Laura F Dagley
- ORCiD
- The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology, University of Melbourne, Melbourne, Australia
- Kenneth Hsu
- Children's Cancer Research Unit, The Children's Hospital at Westmead, Sydney, Australia
- Simone Rizzetto
- ORCiD
- School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia; The Kirby Institute for Infection and Immunity in Society, UNSW, Sydney, Australia
- Jerzy Zieba
- School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia; Neuroscience Research Australia (NeuRA), Randwick, Australia
- Gregory Rice
- Department of Statistics and Actuarial Science, University of Waterloo, Waterloo, Canada
- Victoria Prior
- ORCiD
- Children's Cancer Research Unit, The Children's Hospital at Westmead, Sydney, Australia; Discipline of Child and Adolescent Health, University of Sydney, Sydney, Australia
- Geraldine M O'Neill
- Children's Cancer Research Unit, The Children's Hospital at Westmead, Sydney, Australia; Discipline of Child and Adolescent Health, University of Sydney, Sydney, Australia
- Richard J Williams
- Biofab3D, St. Vincent’s Hospital, Melbourne, Australia; Institute for Innovation in Mental and Physical Health and Clinical Translation (iMPACT), School of Medicine, Deakin University, Victoria, Australia
- David R Nisbet
- Biofab3D, St. Vincent’s Hospital, Melbourne, Australia; Advanced Biomaterials Lab, Research School of Engineering, ANU, Canberra, Australia
- Belinda Kramer
- Children's Cancer Research Unit, The Children's Hospital at Westmead, Sydney, Australia
- Andrew I Webb
- The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology, University of Melbourne, Melbourne, Australia
- Fabio Luciani
- School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia; The Kirby Institute for Infection and Immunity in Society, UNSW, Sydney, Australia
- Mark N Read
- School of Computer Science, Westmead Initiative, and Charles Perkins Centre, University of Sydney, Sydney, Australia
- Maté Biro
- ORCiD
- EMBL Australia, Single Molecule Science node, University of New South Wales, Sydney, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, Australia
- DOI
- https://doi.org/10.7554/eLife.56554
- Journal volume & issue
-
Vol. 9
Abstract
Cytotoxic T lymphocytes (CTLs) are thought to arrive at target sites either via random search or following signals by other leukocytes. Here, we reveal independent emergent behaviour in CTL populations attacking tumour masses. Primary murine CTLs coordinate their migration in a process reminiscent of the swarming observed in neutrophils. CTLs engaging cognate targets accelerate the recruitment of distant T cells through long-range homotypic signalling, in part mediated via the diffusion of chemokines CCL3 and CCL4. Newly arriving CTLs augment the chemotactic signal, further accelerating mass recruitment in a positive feedback loop. Activated effector human T cells and chimeric antigen receptor (CAR) T cells similarly employ intra-population signalling to drive rapid convergence. Thus, CTLs recognising a cognate target can induce a localised mass response by amplifying the direct recruitment of additional T cells independently of other leukocytes.
Keywords
