The effect of Lysimachia savranii on the breast canser cells in cell culture conditions compared to normal cells

Abstract

The breast cancer has become a threat to women's lives at an increasing rate. The inadequate treatments of surgical procedures and chemotherapy affect negatively quality of patient life. Therefore, there has been an increase in demand for alternative and supportive agents, especially for the use of herbal products. The purpose of this study is to evaluate the in vitro anti-tumor activity of Lysimachia savranii in breast cancer cell lines compared to normal adipose tissue derived mesenchymal stem cells. The adipose tissue derived mesenchymal stem cells as normal cells with MCF-7 and, MDA-MB-231 breast cancer cells were cultured. IC50 doses of Lysimachia savranii extract for each cells were determined via MTT assay. The immunocytochemical staining was performed for the oxidative stress by eNOS, whereas apoptosis were analyzed by TUNEL method. The data exhibited with H-score and apoptotic index. The plant extract inhibited the proliferation and survival in the breast cancer cells compared to the stem cells. Lysimachia savranii extract caused the increase of eNOS staining for oxidative stress and the TUNEL labeling for apoptosis. The further molecular techniques should be used to determine the biologic activity of Lysimachia savranii extract and its content should be investigated.

Keywords

• lysimachia savranii
• breast cancer
• proliferation
• cytotoxicity