RNase III-CLASH of multi-drug resistant Staphylococcus aureus reveals a regulatory mRNA 3′UTR required for intermediate vancomycin resistance

Daniel G. Mediati; Julia L. Wong; Wei Gao; Stuart McKellar; Chi Nam Ignatius Pang; Sylvania Wu; Winton Wu; Brandon Sy; Ian R. Monk; Joanna M. Biazik; Marc R. Wilkins; Benjamin P. Howden; Timothy P. Stinear; Sander Granneman; Jai J. Tree

doi:10.1038/s41467-022-31177-8

Nature Communications (Jun 2022)

RNase III-CLASH of multi-drug resistant Staphylococcus aureus reveals a regulatory mRNA 3′UTR required for intermediate vancomycin resistance

Daniel G. Mediati,
Julia L. Wong,
Wei Gao,
Stuart McKellar,
Chi Nam Ignatius Pang,
Sylvania Wu,
Winton Wu,
Brandon Sy,
Ian R. Monk,
Joanna M. Biazik,
Marc R. Wilkins,
Benjamin P. Howden,
Timothy P. Stinear,
Sander Granneman,
Jai J. Tree

Affiliations

Daniel G. Mediati: School of Biotechnology and Biomolecular Sciences, University of New South Wales
Julia L. Wong: School of Biotechnology and Biomolecular Sciences, University of New South Wales
Wei Gao: Department of Microbiology and Immunology, Peter Doherty Institute, University of Melbourne
Stuart McKellar: Centre for Systems and Synthetic Biology, University of Edinburgh
Chi Nam Ignatius Pang: School of Biotechnology and Biomolecular Sciences, University of New South Wales
Sylvania Wu: School of Biotechnology and Biomolecular Sciences, University of New South Wales
Winton Wu: School of Biotechnology and Biomolecular Sciences, University of New South Wales
Brandon Sy: School of Biotechnology and Biomolecular Sciences, University of New South Wales
Ian R. Monk: Department of Microbiology and Immunology, Peter Doherty Institute, University of Melbourne
Joanna M. Biazik: Electron Microscopy Unit, University of New South Wales
Marc R. Wilkins: School of Biotechnology and Biomolecular Sciences, University of New South Wales
Benjamin P. Howden: Department of Microbiology and Immunology, Peter Doherty Institute, University of Melbourne
Timothy P. Stinear: Department of Microbiology and Immunology, Peter Doherty Institute, University of Melbourne
Sander Granneman: Centre for Systems and Synthetic Biology, University of Edinburgh
Jai J. Tree: School of Biotechnology and Biomolecular Sciences, University of New South Wales

DOI: https://doi.org/10.1038/s41467-022-31177-8
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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Regulatory small RNA (sRNA) interact with mRNAs to regulate their stability, transcription, and translation via diverse mechanisms. Here, Mediati et al. apply RNase III-CLASH to multidrug-resistant Staphylococcus aureus to characterise the network of RNA–RNA interactions associated with RNase III and identify a regulatory mRNA 3′UTR, named vigR-3′UTR, involved in the regulation of genes relevant for vancomycin sensitivity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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