LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution

Florian Lindner; Bailey Milne-Davies; Katja Langenfeld; Thorsten Stiewe; Andreas Diepold

doi:10.1038/s41467-020-16169-w

Nature Communications (May 2020)

LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution

Florian Lindner,
Bailey Milne-Davies,
Katja Langenfeld,
Thorsten Stiewe,
Andreas Diepold

Affiliations

Florian Lindner: Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology
Bailey Milne-Davies: Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology
Katja Langenfeld: Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology
Thorsten Stiewe: Institute of Molecular Oncology, Member of the German Center for Lung Research (DZL), Philipps-University
Andreas Diepold: Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology

DOI: https://doi.org/10.1038/s41467-020-16169-w
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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The type III secretion system (T3SS) of bacteria can be used to inject cargo into eukaryotic cells but its lack of target specificity is a disadvantage. Here the authors place the T3SS under the regulation of light by engineering optogenetic switches into the dynamic cytosolic T3SS component SctQ.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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